Steroid hormones analysis with surface-assisted laser desorption/ionization mass spectrometry using catechin-modified titanium dioxide nanoparticles

► Catechin-modified TiO2 NPs as matrices to analyze four steroid hormones by SALDI-MS. ► Effects of NP and catechin concentration, and laser fluence have been investigated. ► This approach provides good quantitative linearity for the analytes. ► It is successful for determining the level of testosterone in a urine sample.