Combining UHPLC and high-resolution MS: A viable approach for the analysis of complex samples?

The coupling of ultra-high-performance liquid chromatography (UHPLC) with high-resolution mass spectrometry (HRMS) has been well received within the analytical community. Both technologies have experienced significant advances in recent years. Not only have the resolution power and sensitivity improved, but the increased robustness, which includes prolonged column lifetime, extended dynamic range, easier mass calibration, and enhanced software handling capabilities, is making this coupling more attractive to a larger user base. In this article, we discuss possibilities and current limitations of the UHPLC-HRMS coupling. We also review the application of UHPLC-HRMS in a variety of fields, where it has been widely accepted or where we anticipate more extensive use in the near future.