The effect of lipids on the enzymatic activity of 6-phosphofructo-1-kinase from B. stearothermophilus

► BsPFK activity increases with lipid vesicle concentration in vitro. ► Lipid vesicles increase the Vmax of BsPFK by a factor of up to 2.5 Km is unchanged. ► BsPFK has a very low association constant with lipid vesicles. ► A BsPFK, lipid monomer interaction increases Vmax activity. ► Kinetic models suggest BsPFK abstracts lipid monomers from vesicles.