کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7693047 | 1496325 | 2010 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Physicochemical characteristics associated with transfection of cationic cholesterol-based gene delivery vectors in the presence of DOPE
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کلمات کلیدی
EMEMhuman cervical carcinoma cellsONPGDC-CholDOTAPLipoplexDMEM1,2-dioleoyl-sn-glycero-3-phosphoethanolamine - 1،2-dioleoyl-sn-glycero-3-phosphoethanolamine3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide - 3- (4،5-dimethylthiazol-2-yl) -2،5-difenyltetrazolium bromideDulbecco's modified Eagle's medium - Medal of Eagle اصلاح شده DulbeccoMTT - MTTO-nitrophenyl-β-D-galactopyranoside - O-nitrofenyl-β-D galactopyranosideCationic lipid - لیپیدهای کاتیونیCationic lipids - لیپیدهای کاتیونیMeS - مسMonolayer - مونولایرZeta potential - پتانسیل زتاSurface potential - پتانسیل سطحDOPE - پیش بینی کردنGene therapy - ژن درمانیHela - کامل
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی (عمومی)
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
The physicochemical properties of a novel series of cholesterol-based cationic lipids in the presence of DOPE were studied by various techniques in an effort to correlate cationic lipid structure with transfection efficacy. It was found that while DOPE improves the β-gal activity of the active AC and MC derivatives, the overall zeta potential of the particles, pDNA complexation and condensation is not improved. This is in stark contrast with the tertiary amine derivative DC whose dispersion properties were improved and its monolayer surface potential is restored at high molecular surface density in the presence of DOPE. Overall the transfection activity mediated by DC and the quaternary ammonium TC derivative was greatly improved in the presence of DOPE and is attributed to decreased cytotoxicity, improved fusogenicity and cellular association.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chemistry and Physics of Lipids - Volume 163, Issue 8, November 2010, Pages 755-764
Journal: Chemistry and Physics of Lipids - Volume 163, Issue 8, November 2010, Pages 755-764
نویسندگان
Molinda D. Kearns, Yesha N. Patel, Michalakis Savva,