Structural and catalytic properties of the peroxygenase P450 enzyme CYP152K6 from Bacillus methanolicus

Bacillus methanolicus CYP152K6 is a novel cytochrome P450 (P450 or CYP) peroxygenase family member. Its tetradecanoic acid-bound crystal structure reveals that the substrate binds through its carboxylate to a conserved arginine residue. Hydrogen peroxide displaces the distal water on the heme, forming a reactive iron-oxo species that catalyzes substrate oxidation.332