کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7783793 | 1500573 | 2018 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Isomalto/malto-polysaccharide structure in relation to the structural properties of starch substrates
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کلمات کلیدی
GPC-MALLSPMAAGlucanotransferase1H NMR - 1H-NMRGC–MS - GC-MSα-glucan - α-گلوکانEnzymatic modification - اصلاح آنزیمیlinkage analysis - تجزیه و تحلیل اتصالdegree of polymerisation - درجه پلیمریزاسیونgas chromatography–mass spectroscopy - طیف سنجی جرم کروماتوگرافی گازStarch - نشاسته proton nuclear magnetic resonance - پروتون مغناطیسی رزونانس هسته ای
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آلی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Isomalto/malto-polysaccharides (IMMPs) are soluble dietary fibres produced by the enzymatic modification of starch with 4,6-α-glucanotransferase (GTFB). The structure, size, and linkage distribution of these IMMPs has remained largely unknown, since most structural information has been based on indirect measurements such as total α-(1 â 6) content, iodine staining and GTFB hydrolytic activity. This study provides a deeper understanding of IMMP structure in relation to its respective starch substrate, by combining preparative fractionation with linkage compositition analysis. IMMPs were produced from a variety of amylose-rich and amylose-free starches. The extent of modification was investigated per IMMP molecular weight (Mw)-fraction, distinguishing between linear α-(1 â 6) linkages introduced by GTFB and starch's native α-(1 â 4,6) branching points. It emerged that the amount of α-(1 â 6) linkages was consistently higher in IMMP low Mw-fractions and that GTFB activity was limited by native α-(1 â 4,6) linkages. The presence of amylose turned out to be a prerequisite for the incorporation of linear α-(1 â 6) linkages in amylopectin.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Carbohydrate Polymers - Volume 185, 1 April 2018, Pages 179-186
Journal: Carbohydrate Polymers - Volume 185, 1 April 2018, Pages 179-186
نویسندگان
P.H. van der Zaal, H.A. Schols, J.H. Bitter, P.L. Buwalda,