A label-free assay for high sensitive detection of RNase based on two near IR fluorescence probes

Two TICT characterize probes (H2 and L2) exhibited strong Near IR luminescence when bound with RNA. The hydrolysis of RNA by RNase resulted in fluorescence quench, causing “OFF-ON-OFF” fluorescence switch. The assay was applied to detect RNase A and RNase H with the detection limit of 1.67 × 10−5 U/mL for RNase A and 1.06 × 10−4 U/mL for RNase H.127