کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8248013 | 1533317 | 2015 | 17 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Space experiment “Cellular Responses to Radiation in Space (CellRad)”: Hardware and biological system tests
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
DLRECDsISSHEKeGFPDDRγH2AXMPUPTFEFBSNF-κBSDSDSBIL-1βataxia telangiectasia mutated - Ataxia telangiectasia جهش یافته استDMSO - DMSOROS - ROSSpace experiment - آزمایش فضاییLET - اجازه دهیدLinear Energy Transfer - انتقال انرژی خطیInternational space station - ایستگاه فضایی بین المللیinterleukin 1β - اینترلوکین 1βtumor necrosis factor α - تومور نکروز عامل αATM - خودپردازDimethyl sulfoxide - دیمتیل سولفواکسیدStorage - ذخیره سازیMicrogravity - ریزگرانش یا میکروگرانشBiocompatibility - زیست سازگاریsodium dodecyl sulphate - سدیم دودسیل سولفاتfetal bovine serum - سرم جنین گاوcytomegalovirus - سیتومگالوویروسCMV - سیتومگالوویروسdouble strand breaks - شکست دو رشتهTNF-α - فاکتور نکروز توموری آلفاnuclear factor κB - فاکتور هسته ای κBGerman aerospace center - مرکز هوافضایی آلمانMicroRNA - میکرو RNA MiRNA - میکروRNA، ریزآرانای، miRNADNA damage response - واکنش به آسیب DNA enhanced green fluorescent protein - پروتئین فلورسنت سبز افزایش یافته استpolytetrafluorethylene - پلی تترافلوئوراتیلنoptical density - چگالی نوریhuman embryonic kidney - کلیه جنین انسانReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
مهندسی و علوم پایه
علوم زمین و سیارات
علوم فضا و نجوم
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The cells will be seeded on ground in multiwell plate units (MPUs), transported to the ISS, and irradiated by an artificial radiation source after an adaptation period at 0Ãg and 1Ãg. After different incubation periods, the cells will be fixed by pumping a formaldehyde solution into the MPUs. Ground control samples will be treated in the same way. For implementation of CellRad in the Biolab on the International Space Station (ISS), tests of the hardware and the biological systems were performed. The sequence of different steps in MPU fabrication (cutting, drilling, cleaning, growth surface coating, and sterilization) was optimized in order to reach full biocompatibility. Different coatings of the foil used as growth surface revealed that coating with 0.1 mg/ml poly-D-lysine supports cell attachment better than collagen type I. The tests of prototype hardware (Science Model) proved its full functionality for automated medium change, irradiation and fixation of cells. Exposure of HEK cells to the β-rays emitted by the radiation source dose-dependently decreased cell growth and increased NF-κB activation. The signal of the fluorescent proteins after formaldehyde fixation was stable for at least six months after fixation, allowing storage of the MPUs after fixation for several months before the transport back to Earth and evaluation of the fluorescence intensity. In conclusion, these tests show the feasibility of CellRad on the ISS with the currently available transport mechanisms.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Life Sciences in Space Research - Volume 7, November 2015, Pages 73-89
Journal: Life Sciences in Space Research - Volume 7, November 2015, Pages 73-89
نویسندگان
Christine E. Hellweg, Shahana Dilruba, Astrid Adrian, Sebastian Feles, Claudia Schmitz, Thomas Berger, Bartos Przybyla, Luca Briganti, Markus Franz, Jürgen Segerer, Luis F. Spitta, Bernd Henschenmacher, Bikash Konda, Sebastian Diegeler,