کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8258483 | 1534608 | 2018 | 34 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
HER4 promotes cell survival and chemoresistance in osteosarcoma via interaction with NDRG1
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کلمات کلیدی
NDRG1Her4FBSDOXMTXTMAOsteosarcoma - استئوسارکوماIHC - ایمونوهیستوشیمیImmunohistochemistry - ایمونوهیستوشیمیDoxorubicin - دوکسوروبیسینCell growth - رشد یاختهCo-IP - شرکت-IPMethotrexate - متوتروکساتChemotherapy resistance - مقاومت شیمی درمانیTissue microarray - میکروآرشی بافتCo-Immunoprecipitation - هم ایمن زداییSphere - کره
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Osteosarcoma (OS) is the most common primary malignant bone tumor in children and adolescents. The abilities of chemotherapy resistance are major roadblock in the successful treatment of OS. The clarification of mechanism regarding cell survival during OS chemotherapy are important. Here, we examined HER4 expression by immunohistochemistry in a large series of OS tissues, and found HER4 expression correlated with tumor characteristics and patient survival rates. HER4 knockdown by shRNA inhibited OS cell growth and tumorigenesis, and induced cell senescence and apoptosis in vitro and in vivo. We demonstrated that HER4 expression upregulated in the adverse conditions, such as serum starvation and sphere culture. Moreover, HER4 knockdown cells became more sensitive in stressful conditions such as loss of attachment, cytotoxic agents or nutrition insufficiency. Mechanism studies revealed that HER4 interacted with NDRG1, and NDRG1 overexpression could antagonize HER4 knockdown-mediated cell growth and apoptosis in stressed conditions. There was a positive correlation between HER4 and NDRG1 immunoreactivity in OS patients. Together, our present study shows that HER4 and/or NDRG1 might play a critical role for the cell survival and chemo-resistance of OS, and could be used as potential therapeutic targets in OS.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease - Volume 1864, Issue 5, Part A, May 2018, Pages 1839-1849
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease - Volume 1864, Issue 5, Part A, May 2018, Pages 1839-1849
نویسندگان
Hongsheng Wang, Wei Sun, Mengxiong Sun, Zeze Fu, Chenghao Zhou, Chongren Wang, Dongqing Zuo, Zifei Zhou, Gangyang Wang, Tao Zhang, Jing Xu, Jian Chen, Zhuoying Wang, Fei Yin, Zhenfeng Duan, Francis J. Hornicek, Zhengdong Cai, Yingqi Hua,