کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8259719 | 1534643 | 2015 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
C/EBP homologous protein (CHOP) gene deficiency attenuates renal ischemia/reperfusion injury in mice
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
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چکیده انگلیسی
C/EBP homologous protein (CHOP), a transcription factor for the expression of apoptosis-related genes, plays an important role in endoplasmic reticulum (ER) stress-related organ diseases, including diseases of the kidney. Here, we investigated the role of CHOP in ischemia/reperfusion (I/R)-induced acute kidney injury using CHOP-knockout (CHOPâ/â) and wild type (CHOP+/+) mice. Fifteen or thirty minutes of bilateral renal ischemia (I/R) insult resulted in necrotic and apoptotic tubular epithelial cell death, together with increases in plasma creatinine (PCr) and blood urea nitrogen (BUN) concentrations. After I/R, BiP/GRP78 and CHOP expressions in the kidney gradually increased over time. CHOP expression was greater in the outer medulla than that in the cortex and localized intensely in the nucleus. I/R caused apoptosis of tubular epithelial cells in both CHOPâ/â and CHOP+/+ mice. The number of apoptotic cells after I/R was lower in CHOPâ/â mice than that in CHOP+/+ mice. Consistent with the degree of apoptosis, I/R-induced kidney morphological and functional damages were milder in CHOPâ/â than that in CHOP+/+ mice. The cleavage of procaspase-3 and the induction of Bax protein after I/R were lower in CHOPâ/â than that in CHOP+/+ mice. In contrast, the expression levels of Bcl-2, Bcl-xL, cIAP2, Mcl-1, and XIAP were higher in CHOPâ/â than that in CHOP+/+ mice. These results indicate that I/R induces ER stress, leading to the activation of CHOP-associated apoptosis signals, resulting in renal functional and histological damages.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease - Volume 1852, Issue 9, September 2015, Pages 1895-1901
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease - Volume 1852, Issue 9, September 2015, Pages 1895-1901
نویسندگان
Mi Ra Noh, Jee In Kim, Sang Jun Han, Tae-Jin Lee, Kwon Moo Park,