کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8262996 | 1534869 | 2015 | 84 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Metabolome and proteome changes with aging in Caenorhabditis elegans
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کلمات کلیدی
C. elegansCaenorhabditis - CaenorhabditisS-adenosyl methionine - S-آدنوزیل متیونینAscorbate - آسکوربات یا ویتامین ثAging - سالخوردگیSorbitol - سوربیتولLifespan - طول عمرSERCA - قلبMetabolome - متابولومMetabolomics - متابولومیکMethyltransferase - متیل ترانسفرازMethylation - متیلاسیونMethionine - متیونینProteome - پروتئومProteomics - پروتئومیکسPurine - پورینPyrimidine - پیریمیدین
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
To expand the understanding of aging in the model organism Caenorhabditis elegans, global quantification of metabolite and protein levels in young and aged nematodes was performed using mass spectrometry. With age, there was a decreased abundance of proteins functioning in transcription termination, mRNA degradation, mRNA stability, protein synthesis, and proteasomal function. Furthermore, there was altered S-adenosyl methionine metabolism as well as a decreased abundance of the S-adenosyl methionine synthetase (SAMS-1) protein. Other aging-related changes included alterations in free fatty acid levels and composition, decreased levels of ribosomal proteins, decreased levels of NADP-dependent isocitrate dehydrogenase (IDH1), a shift in the cellular redox state, an increase in sorbitol content, alterations in free amino acid levels, and indications of altered muscle function and sarcoplasmic reticulum Ca2Â + homeostasis. There were also decreases in pyrimidine and purine metabolite levels, most markedly nitrogenous bases. Supplementing the culture medium with cytidine (a pyrimidine nucleoside) or hypoxanthine (a purine base) increased lifespan slightly, suggesting that aging-induced alterations in ribonucleotide metabolism affect lifespan. An age-related increase in body size, lipotoxicity from ectopic yolk lipoprotein accumulation, a decline in NAD+ levels, and mitochondrial electron transport chain dysfunction may explain many of these changes. In addition, dietary restriction in aged worms resulting from sarcopenia of the pharyngeal pump likely decreases the abundance of SAMS-1, possibly leading to decreased phosphatidylcholine levels, larger lipid droplets, and ER and mitochondrial stress. The complementary use of proteomics and metabolomics yielded unique insights into the molecular processes altered with age in C. elegans.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Gerontology - Volume 72, December 2015, Pages 67-84
Journal: Experimental Gerontology - Volume 72, December 2015, Pages 67-84
نویسندگان
Neil Copes, Clare Edwards, Dale Chaput, Mariam Saifee, Iosif Barjuca, Daniel Nelson, Alyssa Paraggio, Patrick Saad, David Lipps, Stanley M. Jr, Patrick C. Bradshaw,