کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8270065 | 1534969 | 2014 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Nonenzymatic oxygenated metabolites of α-linolenic acid B1- and L1-phytoprostanes protect immature neurons from oxidant injury and promote differentiation of oligodendrocyte progenitors through PPAR-γ activation
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کلمات کلیدی
NeuroblastsPDGFPhytoPALAbFGFMAP2TMREMMPPPAR-γ3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide - 3- (4،5-dimethylthiazol-2-yl) -2،5-difenyltetrazolium bromideMTT - MTTα-linolenic acid - اسید α-لینولنیکArachidonic acid - اسید آراشیدونیکdocosahexaenoic acid - اسید داکوزاگزوائونیکPolyunsaturated fatty acid - اسید چرب غیر اشباعPUFA - اسید چرب چند غیراشباعFatty acids - اسیدهای چربOligodendrocyte - الیگودندروسیتisoprostane - ایزوپروستانIsop - ایسپCrystal violet - بنفش کریستال، کریستال ویولهtetramethylrhodamine ethyl ester perchlorate - تترامتیل رودامین اتیل استر پرکلراتOxidative stress - تنش اکسیداتیوDHA - دوکوساهگزائنوئیک اسیدFree radicals - رادیکال آزادRetinoic acid - رتینوئیک اسیدbasic fibroblast growth factor - فاکتور رشد فیبروبلاست پایهphytoprostane - فیتوپروستانNeuroprotection - محافظت نورونی یا محافظت از عصبMyelin - میلینNeuroprostane - نوروپروتستینNeurop - نورپورMitochondrial inner membrane potential - پتانسیل غشای داخلی میتوکندریایmicrotubule-associated protein 2 - پروتئین مرتبط با میکروتوبول 2oligodendrocyte progenitor - پیشگام اولیگودندروسیتProgenitors - پیشگامانperoxisome proliferator-activated receptor γ - گیرنده پروتئین کننده پروکسیوم فعال γoligodendrocytes - یاخته کم شاخه، الیگودندروسیت
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Phytoprostanes (PhytoP's) are formed in higher plants from α-linolenic acid via a nonenzymatic free radical-catalyzed pathway and act as endogenous mediators capable of protecting cells from damage under various conditions related to oxidative stress. Humans are exposed to PhytoP's, as they are present in relevant quantities in vegetable food and pollen. The uptake of PhytoP's through the olfactory epithelium of the nasal mucosa, upon pollen grain inhalation, is of interest as the intranasal pathway is regarded as a direct route of communication between the environment and the brain. On this basis, we sought to investigate the potential activities of PhytoP's on immature cells of the central nervous system, which are particularly susceptible to oxidative stress. In neuroblastoma SH-SY5Y cells, used as a model for undifferentiated neurons, B1-PhytoP's, but not F1-PhytoP's, increased cell metabolic activity and protected them from oxidant damage caused by H2O2. Moreover, B1-PhytoP's induced a moderate depolarization of the mitochondrial inner membrane potential. These effects were prevented by the PPAR-γ antagonist GW9662. When SH-SY5Y cells were induced to differentiate toward a more mature phenotype, they became resistant to B1-PhytoP activities. B1-PhytoP's also influenced immature cells of an oligodendroglial line, as they increased the metabolic activity of oligodendrocyte progenitors and strongly accelerated their differentiation to immature oligodendrocytes, through mechanisms at least partially dependent on PPAR-γ activity. However, B1-PhytoP's did not protect oligodendrocyte progenitors against oxidant injury. Taken together, these data suggest that B1-PhytoP's, through novel mechanisms involving PPAR-γ, can specifically affect immature brain cells, such as neuroblasts and oligodendrocyte progenitors, thereby conferring neuroprotection against oxidant injury and promoting myelination.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 73, August 2014, Pages 41-50
Journal: Free Radical Biology and Medicine - Volume 73, August 2014, Pages 41-50
نویسندگان
Luisa Minghetti, Rachele Salvi, Maria Lavinia Salvatori, Maria Antonietta Ajmone-Cat, Chiara De Nuccio, Sergio Visentin, Valérie Bultel-Poncé, Camille Oger, Alexandre Guy, Jean-Marie Galano, Anita Greco, Antonietta Bernardo, Thierry Durand,