کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8271767 | 1534990 | 2011 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
2-(2-Fluorobenzamido)benzoate ethyl ester (EFB-1) inhibits superoxide production by human neutrophils and attenuates hemorrhagic shock-induced organ dysfunction in rats
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کلمات کلیدی
fMLPPKCGPCRpKaPDEMODSPAPGE1WST-1IBMXCytochalasin Bt/h3-isobutyl-1-methylxanthine - 3-ایزوبوتیل-1-متیلکسانتینcAMP - cAMPMPO - DFOG-protein-coupled receptor - G-پروتئین گیرندهPMA - LDC هاO2•− - O2 • -ROS - ROScyclic adenosine 3′,5′-monophosphate - آدنوزین cyclic 3 '، 5'-monophosphateadenylate cyclase - آدنیلات سیکلاسsuperoxide anion - آنیون سوپر اکسیدAkt - آکتMultiple organ dysfunction - اختلال عملکرد چندگانهScintillation proximity assay - تست نزدیکی ScintillationFree radicals - رادیکال آزادSOD - سدSuperoxide dismutase - سوکسوکس دیسموتازphorbol myristate acetate - فروبل مریستات استاتPhosphodiesterase - فسفو دی استرازlactate dehydrogenase - لاکتات دهیدروژناز LDH - لاکتات دهیدروژناز به صورت مختصر شده LDH myeloperoxidase - میلوپراکسیداز Neutrophils - نوتروفیل هاprotein kinase A - پروتئین کیناز Aprotein kinase B - پروتئین کیناز BProtein kinase C - پروتئین کیناز سیProstaglandin E1 - پروستاگلاندین E1Reactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: 2-(2-Fluorobenzamido)benzoate ethyl ester (EFB-1) inhibits superoxide production by human neutrophils and attenuates hemorrhagic shock-induced organ dysfunction in rats 2-(2-Fluorobenzamido)benzoate ethyl ester (EFB-1) inhibits superoxide production by human neutrophils and attenuates hemorrhagic shock-induced organ dysfunction in rats](/preview/png/8271767.png)
چکیده انگلیسی
Neutrophil activation after trauma-hemorrhagic shock (T/H) has been implicated in the development of multiple organ dysfunction (MOD). In this study, we report that a small chemical compound, 2-(2-fluorobenzamido)benzoic acid ethyl ester (EFB-1), exhibited a potent inhibitory effect on the formyl-l-methionyl-l-leucyl-l-phenylalanine (FMLP)-induced superoxide anion (O2
- â) release and CD11b expression by human neutrophils. Additionally, administration of EFB-1 in rats subjected to T/H caused a significant improvement in MOD. EFB-1 treatment induced an increase in cAMP formation and protein kinase (PK) A activity in FMLP-activated neutrophils, which occurred through the selective inhibition of cAMP-specific phosphodiesterase (PDE) activity but not an increase in adenylate cyclase function or cGMP-specific PDE activity. FMLP-induced phosphorylation of protein kinase B (AKT), but not calcium mobilization, was reduced by EFB-1. The inhibitory effects of EFB-1 on O2
- â production, CD11b expression, and AKT phosphorylation were reversed by PKA inhibitors (H89 and KT5720). Significantly, administration of EFB-1 (1Â mg/kg body wt) attenuated the myeloperoxidase activity of the intestines, lungs, and liver and reduced the wet/dry weight ratio of the intestines and lungs and plasma alanine aminotransferase and aspartate aminotransferase levels in Sprague-Dawley rats after T/H. Therefore, EFB-1 is a new inhibitor of cAMP-specific PDE that potently suppresses O2
- â release and CD11b expression by human neutrophils and attenuates T/H-induced MOD in rats.
- â) release and CD11b expression by human neutrophils. Additionally, administration of EFB-1 in rats subjected to T/H caused a significant improvement in MOD. EFB-1 treatment induced an increase in cAMP formation and protein kinase (PK) A activity in FMLP-activated neutrophils, which occurred through the selective inhibition of cAMP-specific phosphodiesterase (PDE) activity but not an increase in adenylate cyclase function or cGMP-specific PDE activity. FMLP-induced phosphorylation of protein kinase B (AKT), but not calcium mobilization, was reduced by EFB-1. The inhibitory effects of EFB-1 on O2
- â production, CD11b expression, and AKT phosphorylation were reversed by PKA inhibitors (H89 and KT5720). Significantly, administration of EFB-1 (1Â mg/kg body wt) attenuated the myeloperoxidase activity of the intestines, lungs, and liver and reduced the wet/dry weight ratio of the intestines and lungs and plasma alanine aminotransferase and aspartate aminotransferase levels in Sprague-Dawley rats after T/H. Therefore, EFB-1 is a new inhibitor of cAMP-specific PDE that potently suppresses O2
- â release and CD11b expression by human neutrophils and attenuates T/H-induced MOD in rats.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 50, Issue 12, 15 June 2011, Pages 1737-1748
Journal: Free Radical Biology and Medicine - Volume 50, Issue 12, 15 June 2011, Pages 1737-1748
نویسندگان
Huang-Ping Yu, Pei-Wen Hsieh, Yi-Ju Chang, Pei-Jen Chung, Liang-Mou Kuo, Tsong-Long Hwang,