کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8288579 | 1536258 | 2018 | 36 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Dominant and sensitive control of oxidative flux by the ATP-ADP carrier in human skeletal muscle mitochondria: Effect of lysine acetylation
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کلمات کلیدی
CarboxyatractylosideANTCATMichaelis-Menten - Michaelis به-منتنAcetylation - استیل شدنadenine nucleotide translocase - ترانسکوز نوکلئوتید آدنینNonequilibrium thermodynamics - ترمودینامیکی ناهمگنNET - خالصBioenergetics - زیستانرژی Hill coefficient - ضریب هیلPhosphocreatine - فسفریکراتینEnergy metabolism - متابولیسم انرژیRate of oxygen consumption - میزان مصرف اکسیژنPCR - واکنش زنجیرهٔ پلیمرازcreatine - کراتینCreatine kinase - کراتین کینازFlux control - کنترل شار
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The adenine nucleotide translocase (ANT) of the mitochondrial inner membrane exchanges ADP for ATP. Mitochondria were isolated from human vastus lateralis muscle (nâ¯=â¯9). Carboxyatractyloside titration of O2 consumption rate (Jo) at clamped [ADP] of 21â¯Î¼M gave ANT abundance of 0.97â¯Â±â¯0.14â¯nmol ANT/mg and a flux control coefficient of 82%â¯Â±â¯6%. Flux control fell to 1%â¯Â±â¯1% at saturating (2â¯mM) [ADP]. The KmADP for Jo was 32.4â¯Â±â¯1.8â¯Î¼M. In terms of the free (â3) ADP anion this KmADP was 12.0â¯Â±â¯0.7â¯Î¼M. A novel luciferase-based assay for ATP production gave KmADP of 13.1â¯Â±â¯1.9 μM in the absence of ATP competition. The free anion KmADP in this case was 2.0â¯Â±â¯0.3â¯Î¼M. Targeted proteomic analyses showed significant acetylation of ANT Lysine23 and that ANT1 was the most abundant isoform. Acetylation of Lysine23 correlated positively with KmADP, râ¯=â¯0.74, Pâ¯=â¯0.022. The findings underscore the central role played by ANT in the control of oxidative phosphorylation, particularly at the energy phosphate levels associated with low ATP demand. As predicted by molecular dynamic modeling, ANT Lysine23 acetylation decreased the apparent affinity of ADP for ANT binding.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Archives of Biochemistry and Biophysics - Volume 647, 1 June 2018, Pages 93-103
Journal: Archives of Biochemistry and Biophysics - Volume 647, 1 June 2018, Pages 93-103
نویسندگان
W.T. Willis, D. Miranda-Grandjean, J. Hudgens, E.A. Willis, J. Finlayson, E.A. De Filippis, R. Zapata Bustos, P.R. Langlais, C. Mielke, L.J. Mandarino,