Stability and binding of the phosphorylated species of the N-terminal domain of enzyme I and the histidine phosphocarrier protein from the Streptomyces coelicolor phosphoenolpyruvate:sugar phosphotransferase system

► The phosphorylated species have a lower stability than the unphosphorylated ones. ► Binding of EIsc and HPrsc involves local conformational changes in both proteins. ► Binding between both proteins is entropically driven. ► The complex with the weakest affinity is that between the phosphorylated species. ► The dimerization of the C terminus of EIsc affects binding of HPrsc.