کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8293760 | 1536747 | 2018 | 25 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Steady-state structural fluctuation is a predictor of the necessity of pausing-mediated co-translational folding for small proteins
ترجمه فارسی عنوان
نوسان ساختاری حالت پایدار، پیش بینی کننده ضرورت کشیدن دو طرفه برای تکمیل پروتئین های کوچک است
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کلمات کلیدی
توقف موقت، نوسان سازه، حلالیت پروتئین، پروتئین تاشو،
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
چکیده انگلیسی
Translational pausing coordinates protein synthesis and co-translational folding. It is a common factor that facilitates the correct folding of large, multi-domain proteins. For small proteins, pausing sites rarely occurs in the gene body, and the 3â²-end pausing sites are only essential for the folding of a fraction of proteins. The determinant of the necessity of the pausings remains obscure. In this study, we demonstrated that the steady-state structural fluctuation is a predictor of the necessity of pausing-mediated co-translational folding for small proteins. Validated by experiments with 5 model proteins, we found that the rigid protein structures do not, while the flexible structures do need 3â²-end pausings to fold correctly. Therefore, rational optimization of translational pausing can improve soluble expression of small proteins with flexible structures, but not the rigid ones. The rigidity of the structure can be quantitatively estimated in silico using molecular dynamic simulation. Nevertheless, we also found that the translational pausing optimization increases the fitness of the expression host, and thus benefits the recombinant protein production, independent from the soluble expression. These results shed light on the structural basis of the translational pausing and provided a practical tool for industrial protein fermentation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 498, Issue 1, 25 March 2018, Pages 186-192
Journal: Biochemical and Biophysical Research Communications - Volume 498, Issue 1, 25 March 2018, Pages 186-192
نویسندگان
Wenxi Huang, Wanting Liu, Jingjie Jin, Qilan Xiao, Ruibin Lu, Wei Chen, Sheng Xiong, Gong Zhang,