کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8294913 | 1536755 | 2018 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Pde6brd1 mutation modifies cataractogenesis in Foxe3rct mice
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کلمات کلیدی
Discosoma sp. Red fluorescent proteinRetinitis pigmentosaFOXE3DsRedGlutaredoxin 2PRXRD1qRT-PCRRT-PCRcGMPMAFHRPGAPDHGFPPDE6bperiaxinDMEMORFIgGMIPcDNA - cDNAComplementary DNA - DNA تکمیلیDulbecco's modified Eagle Medium - Eagle Medium اصلاح شده DulbeccoBAC - LACquantitative RT-PCR - RT-PCR کمیCataract - آب مرواریدactin beta - آکتین بتاGenetic modifier - اصلاح کننده ژنتیکstandard deviation - انحراف معیارimmunoglobulin G - ایمونوگلوبولین Ganalysis of variance - تحلیل واریانسANOVA - تحلیل واریانس Analysis of varianceTransgenic - تراریختهreverse transcription PCR - رونویسی معکوس PCRActb - عملopen reading frame - قاب خواندن بازcyclic guanosine monophosphate - مونوفسفات گوانوزین چرخه ایhematoxylin-eosin - هماتوکسیلین ائوزینhorse radish peroxidase - پراکسیداز تربچه اسبGreen fluorescence protein - پروتئین فلورسانس سبزbacterial artificial chromosome - کروموزوم مصنوعی باکتریاییglyceraldehyde-3-phosphate dehydrogenase - گلیسرالیدید-3-فسفات دهیدروژناز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The Foxe3rct mutation, which causes early-onset cataracts, is a recessive mutation found in SJL/J mice. A previous study reported that cataract phenotypes are modified by the genetic background of mouse inbred strains and that the Pde6brd1 mutation, which induced degeneration of the photoreceptor cells, is a strong candidate genetic modifier to accelerate the severity of cataractogenesis of Foxe3rct mice. We created congenic mice by transferring a genomic region including the Foxe3rct mutation to the B6 genetic background, which does not carry the Pde6brd1 mutation. In the congenic mice, the cataract phenotypes became remarkably mild, and the development of cataracts was suppressed for a long time. Moreover, we created transgenic mice by injecting BAC clones including the wild-type Pde6b gene into the eggs of SJL-Foxe3rct mice. Although the resistant effect for cataract phenotypes in transgenic mice was less than that in congenic mice, the severity and onset time of cataract phenotypes were clearly improved and delayed, respectively, compared with the phenotypes of the original SJL-Foxe3rct mice. These results clearly show that the development of early-onset cataracts requires at least two mutant alleles of Foxe3rct and Pde6brd1, and another modifier associated with the severity of cataract phenotypes in Foxe3rct mice underlies the genetic backgrounds in mice.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 496, Issue 1, 29 January 2018, Pages 231-237
Journal: Biochemical and Biophysical Research Communications - Volume 496, Issue 1, 29 January 2018, Pages 231-237
نویسندگان
Kenta Wada, Junichi Saito, Midori Yamaguchi, Yuta Seki, Masamune Furugori, Gou Takahashi, Yasumasa Nishito, Hiroshi Matsuda, Hiroshi Shitara, Yoshiaki Kikkawa,