کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8295159 1536757 2018 24 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Structural insights into a StART-like domain in Lam4 and its interaction with sterol ligands
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Structural insights into a StART-like domain in Lam4 and its interaction with sterol ligands
چکیده انگلیسی
Sterols are essential components of cellular membranes and shape their biophysical properties. The recently discovered family of Lipid transfer proteins Anchored at Membrane contact sites (LAMs) has been suggested to carry out intracellular sterol traffic using StART-like domains. Here, we studied the second StART-like domain of Lam4p from S. cerevisiae by NMR. We show that NMR data are consistent with the StART-like domain structure, and that several functionally important regions within the domain exhibit significant conformational dynamics. NMR titration experiments confirm sterol binding to the canonical sterol-binding site and suggest a role of membrane interactions on the thermodynamics and kinetics of sterol binding.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 495, Issue 3, 15 January 2018, Pages 2270-2274
نویسندگان
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