کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8298758 | 1537043 | 2014 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The D1-173 amino acid is a structural determinant of the critical interaction between D1-Tyr161 (TyrZ) and D1-His190 in Photosystem II
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کلمات کلیدی
psbAN-dodecyl-β-maltosideTyrZPPBQChl monomer of P680 on the D1 or D2 side, respectivelyPD1 and PD243HPheoD1Thermosynechococcus elongatusβ-DMPheophytinP680ChlPSIINIRMALDI-TOF2-(N-morpholino) ethanesulfonic acid - 2- (N-مورفولینو) اتان سولفونیک اسیدspectinomycin - اسپکتنیمایسینChlorophyll - سبزینه یا کلروفیلPhotosystem II - فتوسیستم 2matrix-assisted laser desorption/ionization-time of flight - مدت زمان جذب / زمان یونیزاسیون لیزر ماتریس کمک می کندMeS - مسcontinuous wave - موج مداومNear-infrared - نزدیک مادون قرمزProline - پرولینprimary quinone acceptor - گیرنده اولیه کینونsecondary quinone acceptor - گیرنده ثانویه کوینون
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: The D1-173 amino acid is a structural determinant of the critical interaction between D1-Tyr161 (TyrZ) and D1-His190 in Photosystem II The D1-173 amino acid is a structural determinant of the critical interaction between D1-Tyr161 (TyrZ) and D1-His190 in Photosystem II](/preview/png/8298758.png)
چکیده انگلیسی
The main cofactors of Photosystem II (PSII) are borne by the D1 and D2 subunits. In the thermophilic cyanobacterium Thermosynechococcus elongatus, three psbA genes encoding D1 are found in the genome. Among the 344 residues constituting the mature form of D1, there are 21 substitutions between PsbA1 and PsbA3, 31 between PsbA1 and PsbA2, and 27 between PsbA2 and PsbA3. In a previous study (Sugiura et al., J. Biol. Chem. 287 (2012), 13336-13347) we found that the oxidation kinetics and spectroscopic properties of TyrZ were altered in PsbA2-PSII when compared to PsbA(1/3)-PSII. The comparison of the different amino acid sequences identified the residues Cys144 and Pro173 found in PsbA1 and PsbA3, as being substituted in PsbA2 by Pro144 and Met173, and thus possible candidates accounting for the changes in the geometry and/or the environment of the TyrZ/His190 phenol/imidizol motif. Indeed, these amino acids are located upstream of the α-helix bearing TyrZ and between the two α-helices bearing TyrZ and its hydrogen-bonded partner, D1/His190. Here, site-directed mutants of PSII, PsbA3/Pro173Met and PsbA2/Met173Pro, were analyzed using X- and W-band EPR and UV-visible time-resolved absorption spectroscopy. The Pro173Met substitution in PsbA2-PSII versus PsbA3-PSII is shown to be the main structural determinant of the previously described functional differences between PsbA2-PSII and PsbA3-PSII. In PsbA2-PSII and PsbA3/Pro173Met-PSII, we found that the oxidation of TyrZ by P680+â was specifically slowed during the transition between S-states associated with proton release. We thus propose that the increase of the electrostatic charge of the Mn4CaO5 cluster in the S2 and S3 states could weaken the strength of the H-bond interaction between TyrZâ and D1/His190 in PsbA2 versus PsbA3 and/or induce structural modification(s) of the water molecules network around TyrZ.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Bioenergetics - Volume 1837, Issue 12, December 2014, Pages 1922-1931
Journal: Biochimica et Biophysica Acta (BBA) - Bioenergetics - Volume 1837, Issue 12, December 2014, Pages 1922-1931
نویسندگان
Miwa Sugiura, Yui Ozaki, Masato Nakamura, Nicholas Cox, Fabrice Rappaport, Alain Boussac,