کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8301688 | 1537704 | 2016 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The lipid composition of Legionella dumoffii membrane modulates the interaction with Galleria mellonella apolipophorin III
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کلمات کلیدی
dMPCgas liquid chromatography/mass spectrometryGLC/MSMMPEdimyristoylphosphatidylcholineApolipophorin IIIDPPCTLCFITCnuclear magnetic resonance - رزونانس مغناطیسی هستهایFLIM - FL منLC/ESI-MS - LC / ESI-MSNMR - تشدید مغناطیسی هستهای dipalmitoylphosphatidylcholine - دیپالمیتویف فسفاتیدیل کولینFTIR - طیف سنج مادون قرمزFourier transform infrared spectroscopy - طیف سنجی مادون قرمز تبدیل فوریه یا طیف سنجی FTIRMembrane phospholipids - غشاء فسفولیپیدphosphatidylcholine - فسفاتیدیل کولینphosphatidylglycerol - فسفاتیدیل گلیسرولphosphatidylethanolamine - فسفاتیدیلتانولامینfluorescein isothiocyanate - فلوئورسین ایسوتیوسیاناتfluorescence lifetime imaging microscopy - میکروسکوپ تصویربرداری طول عمر فلورسنتCardiolipin - کاردیولیپینthin-layer chromatography - کروماتوگرافی نازک لایه
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Apolipophorin III (apoLp-III), an insect homologue of human apolipoprotein E (apoE), is a widely used model protein in studies on protein-lipid interactions, and anti-Legionella activity of Galleria mellonella apoLp-III has been documented. Interestingly, exogenous choline-cultured Legionella dumoffii cells are considerably more susceptible to apoLp-III than non-supplemented bacteria. In order to explain these differences, we performed, for the first time, a detailed analysis of L. dumoffii lipids and a comparative lipidomic analysis of membranes of bacteria grown without and in the presence of exogenous choline. 31P NMR analysis of L. dumoffii phospholipids (PLs) revealed a considerable increase in the phosphatidylcholine (PC) content in bacteria cultured on choline medium and a decrease in the phosphatidylethanolamine (PE) content in approximately the same range. The interactions of G. mellonella apoLp-III with lipid bilayer membranes prepared from PLs extracted from non- and choline-supplemented L. dumoffii cells were examined in detail by means of attenuated total reflection- and linear dichroism-Fourier transform infrared spectroscopy. Furthermore, the kinetics of apoLp-III binding to liposomes formed from L. dumoffii PLs was analysed by fluorescence correlation spectroscopy and fluorescence lifetime imaging microscopy using fluorescently labelled G. mellonella apoLp-III. Our results indicated enhanced binding of apoLp-III to and deeper penetration into lipid membranes formed from PLs extracted from the choline-supplemented bacteria, i.e. characterized by an increased PC/PE ratio. This could explain, at least in part, the higher susceptibility of choline-cultured L. dumoffii to G. mellonella apoLp-III.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids - Volume 1861, Issue 7, July 2016, Pages 617-629
Journal: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids - Volume 1861, Issue 7, July 2016, Pages 617-629
نویسندگان
Marta PalusiÅska-Szysz, Agnieszka Zdybicka-Barabas, Emilia ReszczyÅska, RafaÅ Luchowski, Magdalena Kania, Nicolas Gisch, Franziska Waldow, PaweÅ Mak, Witold Danikiewicz, WiesÅaw I. Gruszecki, MaÅgorzata CytryÅska,