کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8303576 | 1537822 | 2006 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Hypothyroidism down-regulates mitochondrial citrate carrier activity and expression in rat liver
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کلمات کلیدی
TBS6-n-propyl-2-thiouracilPTUTris buffer solutioniOpACCFAS1,2,3-Benzenetricarboxylic acid - 1،2،3-بنزنتریکاربوکولیک اسیدacetyl-CoA carboxylase - استیل کروکسی سیلازiopanoic acid - اسید آیپانوئیکfatty acid synthase - اسید چرب سنتازFatty acids - اسیدهای چربBtA - بتاPhospholipid - فسفولیپیدLipogenesis - لیپوژنزFAME یا fatty acid methyl esters - متیل استرهای اسید چربMitochondria - میتوکندریاhigh performance liquid chromatography - کروماتوگرافی مایع با کارایی بالاHPLC - کروماتوگرافی مایعی کاراHypothyroidism - کم کاری تیروئید
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Hypothyroidism down-regulates mitochondrial citrate carrier activity and expression in rat liver Hypothyroidism down-regulates mitochondrial citrate carrier activity and expression in rat liver](/preview/png/8303576.png)
چکیده انگلیسی
The effect of hypothyroidism on citrate carrier (CiC) activity has been investigated in rat-liver mitochondria. The rate of citrate transport was reduced by â¼50% in mitochondria from hypothyroid as compared with euthyroid rats. In parallel, a decrease in the rate of de novo fatty acid synthesis was observed in the cytosol of the former animals. Kinetic analysis of citrate transport revealed that only the Vmax was reduced by hypothyroidism, while Km was almost unaffected. Hypothyroidism increased the mitochondrial percentage of phosphatidylcholine while decreased that of phosphatidylethanolamine; an altered fatty acid pattern but no significant difference in the sum of saturated and unsaturated fatty acids as well as in the unsaturation index was observed. The CiC Arrhenius plot did not show appreciable difference between the two groups of rats. However, Western blot analysis associated with mRNA quantitation indicated that both protein level and mRNA accumulation of hepatic CiC were noticeably decreased in hypothyroid state. Therefore, a reduced content of the carrier protein can represent a plausible mechanism to explain the decline in the CiC activity observed in rat liver mitochondria of hypothyroid rats.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids - Volume 1761, Issue 4, April 2006, Pages 484-491
Journal: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids - Volume 1761, Issue 4, April 2006, Pages 484-491
نویسندگان
Anna M. Giudetti, Monica Leo, Luisa Siculella, Gabriele V. Gnoni,