کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8304130 | 1538381 | 2018 | 39 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Diacylglycerol kinase δ controls down-regulation of cyclin D1 for C2C12 myogenic differentiation
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کلمات کلیدی
PC-PLCPBSDMEMFBSDG kinaseCyclin D1DGKPKCmTOREGFRDulbecco's modified Eagle's medium - Medal of Eagle اصلاح شده Dulbeccophosphatidic acid - اسید فسفاتیدیکMyogenic differentiation - تمایز میوگنdiacylglycerol kinase - دیسیل گلیسرول کینازdiacylglycerol - دیسیل گلیسیرینMyosin heavy chain - زنجیره سنگین میوزینfetal bovine serum - سرم جنین گاوSkeletal muscle - عضله اسکلتیphosphatidylinositol - فسفاتیدیل اینوزیتولPhosphatidylcholine-specific phospholipase C - فسفاتیدیل کولین خاص فسفولیپاز CMHC - مجموعه سازگاری بافتی اصلیPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریProtein kinase C - پروتئین کیناز سیEpidermal growth factor receptor - گیرنده فاکتور رشد اپیدرمال
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Diacylglycerol kinase (DGK) is a lipid-metabolizing enzyme that phosphorylates diacylglycerol (DG) to produce phosphatidic acid (PA). DGKδ is highly expressed in the skeletal muscle, and a decrease in DGKδ expression increases the severity of type 2 diabetes. However, the role of DGKδ in myogenic differentiation is still unknown. The present study demonstrated that DGKδ expression was down-regulated in the early stage of C2C12 myogenic differentiation almost concurrently with a decrease in cyclin D1 expression. The knockdown of DGKδ by DGKδ-specific siRNAs significantly increased the levels of cyclin D1 expression at 48â¯h after C2C12 myogenic differentiation. In contrast, at the same time, the knockdown of DGKδ decreased the levels of myogenin expression and the number of myosin heavy chain (MHC)-positive cells. These results indicate that DGKδ regulates the early differentiation of C2C12 myoblasts via controlling the down-regulation of cyclin D1 expression. Moreover, the suppression of DGKδ expression increased the phosphorylation levels of conventional and novel protein kinase Cs (cnPKCs). Furthermore, DGKδ suppression increased the levels of cyclin D1 and phospho-cnPKCs even at the first 24â¯h of myogenic differentiation. These results suggest that DGKδ controls the down-regulation of cyclin D1 expression by attenuating the PKC signaling pathway for C2C12 myogenic differentiation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimie - Volume 151, August 2018, Pages 45-53
Journal: Biochimie - Volume 151, August 2018, Pages 45-53
نویسندگان
Hiromichi Sakai, Chiaki Murakami, Ken-ichi Matsumoto, Takeshi Urano, Fumio Sakane,