Simple and precise detection of UGT1A1 polymorphisms with a modified loop-hybrid mobility shift assay using Cy5-labeled loop probes

When a loop-hybrid using a Cy5-tagged probe for the ⁎28 locus and ⁎6 locus were combined and used for mobility shift assay, simultaneous typing of the ⁎28 and ⁎6 variants was achieved in a single lane.