کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8318786 | 1539113 | 2018 | 33 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Do differences in the activities of carbohydrate metabolism enzymes between Lake Whitefish ecotypes match predictions from transcriptomic studies?
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
PYGGAPDHTPICoregonus clupeaformisPFKGPiTriosephosphate isomeraseGPDAldo - آلدوAldolase - آلدولازLocal adaptation - سازگاری محلیWhite muscle - عضله سفیدPhosphoglucose isomerase - فسفو گلوکوز ایزومرازPhosphofructokinase - فسفوفروتکتینازlactate dehydrogenase - لاکتات دهیدروژناز LDH - لاکتات دهیدروژناز به صورت مختصر شده LDH pyruvate kinase - پیرووات کینازLiver - کبدCreatine phosphokinase - کراتین فسفوکینازglyceraldehyde 3-phosphate dehydrogenase - گلیسرولیدید 3-فسفات دهیدروژنازGlycolysis - گلیکولیز یا قندکافتglycogen synthase - گلیکوژن سنتازGlycogen phosphorylase - گلیکوژن فسفریلاسGlycogenesis - گلیکوژنزGlycogenolysis - گلیکوژنولیز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Transcriptomic studies are facilitating the search for the molecular bases of adaptation in natural populations, but the impact of these differences in mRNA content on animal physiology are often unknown. One way to determine if molecular changes have the potential to influence animal physiology and performance is to test for correlated changes at higher levels of biological organization, including enzyme activity. Here, we measure the activities of carbohydrate metabolism enzymes to test if previously documented genetic and transcriptomic variation between 'dwarf' and 'normal' Lake Whitefish ecotypes are associated with corresponding changes in enzyme activity (measured as maximal rate, Vmax) in liver and skeletal muscle. We use laboratory-reared fish from the same populations as prior transcriptomic studies and find that white muscle mRNA content is a good predictor of glycolytic and glycogen metabolism enzyme activity, and dwarf whitefish have evolved higher activities than normal whitefish. However, the differences in hepatic mRNA content found between ecotypes in prior studies are not associated with comparable changes in enzyme activity. For example, dwarf whitefish have lower enzyme activities, but higher transcript abundances for two glycolytic enzymes compared to normal whitefish. Overall, we find that transcriptomic studies successfully highlight evolutionary variation in enzyme activities, but not always in the direction predicted, indicating that a variety of tissue-specific regulatory mechanisms contributed to the evolution of energy metabolism in Lake Whitefish.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - Volume 224, October 2018, Pages 138-149
Journal: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - Volume 224, October 2018, Pages 138-149
نویسندگان
Anne C. Dalziel, Martin Laporte, Helga Guderley, Louis Bernatchez,