کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8318973 | 1539253 | 2018 | 40 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Structural and functional analysis of two novel somatostatin receptors identified from topmouth culter (Erythroculter ilishaeformis)
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
pKaSomatostatin 14GnRHSSTRsmRNAsIGFsTMDHEK293GHRHGHRMS-222MicrocystinsNPYDMEMRT-PCRMCsMC-LR17β-estradiol - 17β استرادیولcAMP - cAMPcDNA - cDNADMSO - DMSODulbecco's modified Eagle's medium - Medal of Eagle اصلاح شده DulbeccoCyclic adenosine monophosphate - آدنوزین مونوفسفات Cyclicadenylate cyclase - آدنیلات سیکلاسcomplementary deoxyribonucleic acid - اسید دز اکسید ریبونوکلئیک مکملtransmembrane domain - دامنه فرابنفشDimethyl sulfoxide - دیمتیل سولفواکسیدSomatostatin - سوماتواستاتینinsulin-like growth factors - عوامل رشد انسولین مانندmicrocystin-LR - میکروسیستین LRmap - نقشهgrowth hormone releasing hormone - هورمون آزاد کننده هورمون رشدGonadotropin-releasing hormone - هورمون آزاد کننده گنادوتروپینGrowth hormone - هورمون رشدreverse transcription-polymerase chain reaction - واکنش زنجیره ای رونویسی-پلیمراز معکوسpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازmitogen-activated protein - پروتئین فعال mitogenhuman embryonic kidney 293 - کلیه جنینی انسان 293Somatostatin receptors - گیرنده های سواستوستاتینgrowth hormone receptor - گیرنده هورمون رشدNeuropeptide Y - یوروپروتئین Y
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
In the present study, we cloned and characterized two somatostatin (SS) receptors (SSTRs) from topmouth culter (Erythroculter ilishaeformis) designated as EISSTR6 and EISSTR7. Analysis of EISSTR6 and EISSTR7 signature motifs, 3D structures, and homology with the known members of the SSTR family indicated that the novel receptors had high similarity to the SSTRs of other vertebrates. EISSTR6 and EISSTR7 mRNA expression was detected in 17 topmouth culter tissues, and the highest level was observed in the pituitary. Luciferase reporter assay revealed that SS14 significantly inhibited forskolin-stimulated pCRE-luc promoter activity in HEK293 cells transiently expressing EISSTR6 and EISSTR7, indicating that the receptors can be activated by SS14. We also identified phosphorylation sites important for the functional activity of EISSTR6 and EISSTR7 by mutating Ser23, 43, 107, 196, 311 and Ser7, 29, 61, 222, 225 residues, respectively, to Ala, which significantly reduced the inhibitory effects of SS14 on the CRE promoter mediated by EISSTR6 and EISSTR7. Furthermore, treatment of juvenile topmouth culters with microcystin-LR or 17β-estradiol significantly affected EISSTR6 and EISSTR7 transcription in the brain, liver and spleen, suggesting that these receptors may be involved in the pathogenic mechanisms induced by endocrine disruptors. Our findings should contribute to the understanding of the structure-function relationship and evolution of the SSTR family.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology - Volume 210, August 2018, Pages 18-29
Journal: Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology - Volume 210, August 2018, Pages 18-29
نویسندگان
Haiyan Dong, Yunhai Wei, Chao Xie, Xiaoxuan Zhu, Chao Sun, Qianwen Fu, Lei Pan, Mengting Wu, Yinghan Guo, Jianwei Sun, Hong Shen, Jinyun Ye,