کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8322247 | 1539864 | 2016 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Circumventing the Crabtree Effect: A method to induce lactate consumption and increase oxidative phosphorylation in cell culture
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کلمات کلیدی
carbonyl cyanide p-(trifluoromethoxy) phenylhydrazoneATP5ATOM202-DeoxyglucoseSOD2AMPKSOD1FCCPBCAATP synthase subunit alphaSDHBGAPDHOXPHOSSuccinate dehydrogenase subunit BPCNA2-DG - 2 DGProliferating Cell Nuclear Antigen - آنتیژن هسته ای تکثیر سلولیbicinchoninic acid - بیسینکنینیک اسیدsuperoxide dismutase 1 - سوپر اکسید دیسموتاز 1Superoxide dismutase 2 - سوکسوکس دیسموتاز 2Oxidative phosphorylation - فسفوریلاسیون اکسیداتیوOxidative phosphorylation (OXPHOS) - فسفوریلاسیون اکسیداتیو (OXPHOS)Lactate - لاکتاتMitochondria - میتوکندریاCell culture - کشت سلولglyceraldehyde-3-phosphate dehydrogenase - گلیسرالیدید-3-فسفات دهیدروژناز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Circumventing the Crabtree Effect: A method to induce lactate consumption and increase oxidative phosphorylation in cell culture Circumventing the Crabtree Effect: A method to induce lactate consumption and increase oxidative phosphorylation in cell culture](/preview/png/8322247.png)
چکیده انگلیسی
Most cells grown in glucose-containing medium generate almost all their ATP via glycolysis despite abundant oxygen supply and functional mitochondria, a phenomenon known as the Crabtree effect. By contrast, most cells within the body rely on mitochondrial oxidative phosphorylation (OXPHOS) to generate the bulk of their energy supply. Thus, when utilising the accessibility of cell culture to elucidate fundamental elements of mitochondria in health and disease, it is advantageous to adopt culture conditions under which the cells have greater reliance upon OXPHOS for the supply of their energy needs. Substituting galactose for glucose in the culture medium can provide these conditions, but additional benefit can be gained from alternate in vitro models. Herein we describe culture conditions in which complete autonomous depletion of medium glucose induces a lactate-consuming phase marked by increased MitoTracker Deep Red staining intensity, increased expression of Kreb's cycle proteins, increased expression of electron transport chain subunits, and increased sensitivity to the OXPHOS inhibitor rotenone. We propose these culture conditions represent an alternate accessible model for the in vitro study of cellular processes and diseases involving the mitochondrion without limitations incurred via the Crabtree effect.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The International Journal of Biochemistry & Cell Biology - Volume 79, October 2016, Pages 128-138
Journal: The International Journal of Biochemistry & Cell Biology - Volume 79, October 2016, Pages 128-138
نویسندگان
Alexandra I. Mot, Jeffrey R. Liddell, Anthony R. White, Peter J. Crouch,