کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8323072 | 1539889 | 2014 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Conditioning causes an increase in glucose transporter-4 levels in mononuclear cells in sled dogs
ترجمه فارسی عنوان
تهویه مطبوع سبب افزایش سطح حمل و نقل گلوکز -4 در سلولهای تک هسته ای در سگهای سورتمه می شود
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کلمات کلیدی
GLUT4T2DIACUCHbA1cRPMRPMIHOMA-IRBCAWBCEDTA - اتیلن دی آمین تترا استیک اسید Ethylenediaminetetraacetic acid - اتیلینیدامین تتراستیک اسیدhomeostasis model assessment of insulin resistance - ارزیابی مدل هومیوستاز مقاومت به انسولینbicinchoninic acid - بیسینکنینیک اسیدEnzyme-linked immunosorbent assay - تست الیزاELISA - تست الیزاInsulin sensitivity - حساسیت به انسولینType 2 diabetes - دیابت نوع 2revolutions per minute - سرعت در هر دقیقهMononuclear cells - سلولهای تک هسته ایConditioning - شرطیشدنInsulin resistance - مقاومت به انسولینRoswell Park Memorial Institute medium - موسسه خاطرات Roswell Park mediumGlycated hemoglobin - هموگلوبین گلیکوزیلهexercise - ورزشwhite blood cells - گلبول های سفید خون
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
چکیده انگلیسی
This study was designed to investigate the effects of physical conditioning on the expression of the insulin sensitive glucose transporter-4 protein (GLUT4) on mononuclear cells and HOMA-IR levels in dogs and compared to results reported in human skeletal muscle and the skeletal muscle of rodent models. Blood was sampled from conditioned dogs (n = 8) and sedentary dogs (n = 8). The conditioned dogs were exercised four months prior the experiment and were following a uniform training protocol, whereas the sedentary dogs were not. GLUT4 expression in mononuclear cells and plasma insulin levels were measured using commercially available enzyme-linked immunosorbent assay (ELISA). Blood glucose levels were determined using blood plasma. HOMA-IR was calculated using plasma insulin and blood glucose levels using the linear approximation formula. Our results indicate that the state of conditioning had a significant effect on the GLUT4 expression at the surface of mononuclear cells. HOMA-IR was also affected by conditioning in dogs. GLUT4 levels in mononuclear cells of sled dogs were inversely correlated with the homeostasis model assessment of insulin sensitivity. This study demonstrates that conditioning increases GLUT4 levels in mononuclear cells of sled dogs as it has been previously reported in skeletal muscle. Our results support the potential of white blood cells as a proxy tissue for studying insulin signaling and may lead to development of a minimally invasive and direct marker of insulin resistance. This may be the first report of GLUT4 in mononuclear cells in response to exercise and measured with ELISA.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The International Journal of Biochemistry & Cell Biology - Volume 55, October 2014, Pages 227-231
Journal: The International Journal of Biochemistry & Cell Biology - Volume 55, October 2014, Pages 227-231
نویسندگان
Theresia M. Schnurr, Arleigh J. Reynolds, Sally J. Gustafson, Lawrence K. Duffy, Kriya L. Dunlap,