کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8336805 | 1540642 | 2015 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Maternal gestational betaine supplementation-mediated suppression of hepatic cyclin D2 and presenilin1 gene in newborn piglets is associated with epigenetic regulation of the STAT3-dependent pathway
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کلمات کلیدی
PSEN1Presenilin-1LC3BSTAT3CCND2H3K27me3ULK1GSTP1TRPM7H3K4me3LC3AMeDIPNEAT1Nuclear enriched abundant transcript 1Histone H3 lysine 27 trimethylationCyclin D2PCNAmyristoylated alanine-rich C-kinase substrateunc-51-like kinase 1Bcl-2Presenilin1MARCKSAtg12MGMTMethylated DNA immunoprecipitation - Immunoprecipitation DNA متیل شدهO-6-Methylguanine-DNA Methyltransferase - O-6-Methylguanine-DNA متیل ترانسفرازmicro RNA - RNA میکروProliferating Cell Nuclear Antigen - آنتیژن هسته ای تکثیر سلولیchromatin immunoprecipitation - ایمن سازی کروماتینcon - باBax - باکسlight chain 3 - زنجیره سبک 3BET - شرطB-cell lymphoma-2 - لنفوم سلول B-2signal transducer and activator of transcription 3 - مبدل سیگنال و فعال کننده رونویسی 3Epigenetic regulation - مقررات اپی ژنتیکMiRNA - میکروRNA، ریزآرانای، miRNAHistone H1 - هیستون H1Histone H3 - هیستون H3histone H3 lysine 4 trimethylation - هیستون H3 لیزین 4 trimethylationbody weight - وزن بدنBcl-2 Associated X protein - پروتئین Bcl-2 Associated XCHiP - چیپGlutathione S-transferase P1 - گلوتاتیون S-ترانسفراز P1
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Betaine, which donates methyl groups through methionine metabolism for DNA and protein methylation, is critical for epigenetic gene regulation, especially during fetal development. Here we fed gestational sows with control or betaine supplemented diets (3 g/kg) throughout the pregnancy to explore the effects of maternal betaine on hepatic cell proliferation in neonatal piglets. Neonatal piglets born to betaine-supplemented sows demonstrated a reduction of cell number and DNA content in the liver, which was associated with significantly down-regulated hepatic expression of cell cycle regulatory genes, cyclin D2 (CCND2) and presenilin1 (PSEN1). Moreover, STAT3 binding to the promoter of CCND2 and PSEN1 was also lower in betaine-exposed piglets, accompanied by strong reduction of STAT3 mRNA and protein expression, along with its phosphorylation at Tyr705 and Ser727 residues. Also, prenatal betaine exposure significantly attenuated upstream kinases of STAT3 signaling pathway (phospho-ERK1/2, phospho-SRC and phospho-JAK2) in the livers of neonates. Furthermore, the repressed STAT3 expression in the liver of betaine-exposed piglets was associated with DNA hypermethylation and more enriched repression histone mark H3K27me3 on its promoter, together with significantly up-regulated expression of H3K27me3 and enhancer of zeste homolog 2 (EZH2) proteins, as well as miR-124a, which targets STAT3. Taken together, our results suggest that maternal dietary betaine supplementation during gestation inhibits hepatic cell proliferation in neonatal piglets, at least partly, through epigenetic regulation of hepatic CCND2 and PSEN1 genes via a STAT3-dependent pathway. These neonatal changes in cell cycle and proliferation regulation may lead to lower liver weight and hepatic DNA content at weaning.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The Journal of Nutritional Biochemistry - Volume 26, Issue 12, December 2015, Pages 1622-1631
Journal: The Journal of Nutritional Biochemistry - Volume 26, Issue 12, December 2015, Pages 1622-1631
نویسندگان
Demin Cai, Mengjie Yuan, Yimin Jia, Haoyu Liu, Yun Hu, Ruqian Zhao,