کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8338723 | 1541007 | 2014 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Melatonin counteracts BMP-6 regulation of steroidogenesis by rat granulosa cells
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کلمات کلیدی
P450 steroid side-chain cleavage enzymeBMP type-I receptorBMPRIactivin type-II receptorActRIIactivin type-I receptorBMP type-II receptor3βHSDP450arom3β-Hydroxysteroid dehydrogenaseFSHRP450sccPCOSBMPRIIpKaTGF-βGnRHGDFP450 aromatase - P450 آروماتازROS - ROSALK - آلکSteroidogenesis - استروئیدوژنزActRI - بازیگر نقش اول زنtransforming growth factor - تبدیل فاکتور رشدoocyte - تخمک گذاریStar - ستارهgranulosa cells - سلولهای گرانولوزاPolycystic ovary syndrome - سندرم تخمدان پلی کیستیکGrowth differentiation factor - عامل تمایز رشدfollicle-stimulating hormone (FSH) - فولیکول هورمون تحریک کننده (FSH)BMP - مدیریت فرایند کسب و کارMelatonin - ملاتونینGonadotropin-releasing hormone - هورمون آزاد کننده گنادوتروپینfollicle-stimulating hormone - هورمون تحریک کننده فولیکولluteinizing hormone - هورمون جسم زردFSH - هورمون محرکه فولیکولی Steroidogenic acute regulatory protein - پروتئین حاکم استروئیدوژنیک حادBone morphogenetic protein - پروتئین مورفوژنیک استخوانbone morphogenetic protein (BMP) - پروتئین مورفوژنیک استخوان (BMP)protein kinase A - پروتئین کیناز Aactivin receptor-like kinase - کیناز گیرنده مانند فعال استReactive oxygen species - گونههای فعال اکسیژنFSH receptor - گیرنده FSH
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The ovarian bone morphogenetic protein (BMP) system is a physiological inhibitor of luteinization in growing ovarian follicles. BMP-6, which is expressed in oocytes and granulosa cells of healthy follicles, specifically inhibits FSH actions by suppressing adenylate cyclase activity. In the present study, we studied the role of melatonin in ovarian steroidogenesis using rat primary granulosa cells of immature female rat ovaries by focusing on the interaction with BMP-6 activity. Treatment with melatonin had no direct effect on FSH-induced progesterone or estradiol production by granulosa cells, and the results were not affected by the presence of co-cultured oocytes. In addition, synthesis of cAMP by granulosa cells was not significantly altered by melatonin treatment. To elucidate the interaction between activities of melatonin and BMPs, the effect of melatonin treatment on suppression of progesterone synthesis by BMP-6 was investigated. Interestingly, the inhibitory effect of BMP-6 on FSH-induced progesterone production was impaired by co-treatment with melatonin. Granulosa cells express higher levels of MT1 than MT2, and BMP-6 had no significant effect on MT1 expression in granulosa cells. However, BMP-6-induced Smad1/5/8 phosphorylation and Id-1 transcription were suppressed by melatonin, suggesting that melatonin has an inhibitory effect on BMP receptor signaling in granulosa cells. Although the expression levels of ALK-2, -6, ActRII and BMPRII were not affected by melatonin, inhibitory Smad6, but not Smad7, expression was upregulated by melatonin. Thus, melatonin plays a role in the regulation of BMP-6 signal intensity for controlling progesterone production in the ovary. These findings suggest that the effect of melatonin on maintenance of ovarian function is, at least in part, due to the regulation of endogenous BMP activity in granulosa cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The Journal of Steroid Biochemistry and Molecular Biology - Volume 143, September 2014, Pages 233-239
Journal: The Journal of Steroid Biochemistry and Molecular Biology - Volume 143, September 2014, Pages 233-239
نویسندگان
Eri Nakamura, Fumio Otsuka, Tomohiro Terasaka, Kenichi Inagaki, Takeshi Hosoya, Naoko Tsukamoto-Yamauchi, Kishio Toma, Hirofumi Makino,