کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8339671 | 1541042 | 2010 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Highly sensitive and specific analysis of sterol profiles in biological samples by HPLC-ESI-MS/MS
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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![عکس صفحه اول مقاله: Highly sensitive and specific analysis of sterol profiles in biological samples by HPLC-ESI-MS/MS Highly sensitive and specific analysis of sterol profiles in biological samples by HPLC-ESI-MS/MS](/preview/png/8339671.png)
چکیده انگلیسی
High-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) is a powerful method for the microanalysis of compounds in biological samples. Compared with gas chromatography-mass spectrometry (GC-MS), this method is more broadly applicable to various compounds and usually does not require a derivatization step before analysis. However, when neutral sterols are analyzed, the sensitivities of usual HPLC-MS/MS method are not superior to those of GC-MS because the sterols are relatively resistant to ionization. In this review, we introduce the recent development of HPLC-MS/MS analysis for the quantification of non-cholesterol sterols. By adding an effective derivatization step to the conventional procedure, sterol analysis by HPLC-MS/MS surpassed that obtained by GC-MS in sensitivity. In addition, sufficient specificity of this method was achieved by selected reaction monitoring (SRM) and thorough chromatographic separation of each sterol.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The Journal of Steroid Biochemistry and Molecular Biology - Volume 121, Issues 3â5, August 2010, Pages 556-564
Journal: The Journal of Steroid Biochemistry and Molecular Biology - Volume 121, Issues 3â5, August 2010, Pages 556-564
نویسندگان
Akira Honda, Teruo Miyazaki, Tadashi Ikegami, Junichi Iwamoto, Kouwa Yamashita, Mitsuteru Numazawa, Yasushi Matsuzaki,