کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8344550 | 1541583 | 2018 | 38 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Relation of nNOS isoforms to mitochondrial density and PGC-1alpha expression in striated muscles of mice
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
EDLnNOSNBTPGC-1alphaSDHROS - ROSnitroblue tetrazolium - tetrazolium nitroblueTem - این استTransgenic - تراریختهSol - سلneuronal nitric oxide synthase - سنتاز اکسید نیتریک عصبیsuccinate dehydrogenase - سوکسیناد دهیدروژنازCitrate synthase - سیترات سیتواستاتExtensor digitorum longus muscle - عضلات بازکننده انگشتان لونگوسGastrocnemius muscle - عضله GastrocnemiusSoleus muscle - عضله SoleusSkeletal muscle - عضله اسکلتیTibialis anterior muscle - عضله قدامی تیبالیplantaris muscle - عضله پلاتاریسRectus Femoris muscle - ماهیچه رکتوس فمورسMitochondria - میتوکندریاTransmission electron microscopy - میکروسکوپ الکترونی عبوریknockout - ناکاوتNitric oxide - نیتریک اکسیدNeuronal nitric oxide synthase (nNOS) - نیتریک اکسید سنتاز (nNOS)Reactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The expression of neuronal NO synthase (nNOS) alpha- and beta-isoforms in skeletal muscle is well documented but only little information is available about their regulation/functions. Using different mouse models, we now assessed whether the expression of nNOS-isoforms in muscle fibers is related to mitochondria content/activity and regulated by peroxisome proliferator-activated receptor gamma coactivator-1alpha (PGC-1alpha). Catalytic histochemistry revealed highest nNOS-concentrations to be present in type-2 oxidative muscle fibers. Differences in mitochondrial density between nNOS-KO-mice and WT-littermates established by morphometry after transmission electron microscopy were significant in the oxidative portion of the tibialis anterior muscle (TA) but not in rectus femoris muscle (RF) indicating an nNOS-dependent mitochondrial pool in TA. Quantitative immunoblotting displayed the nNOS alpha-isoform to preponderate in those striated muscles of C57BL/6-mice that comprise of many type-2 oxidative fibers, e.g. TA, while roughly even levels of the two nNOS-isoforms were expressed in those muscles that mainly consist of type-2 glycolytic fibers, e.g. RF. Differences in citrate synthase-activity in muscle homogenates between nNOS-KO-mice and WT-littermates were positively related to nNOS alpha-isoform levels. In transgenic-mice over-expressing muscular PGC-1alpha compared to WT-littermates, immunoblotting revealed a significant shift in nNOS-expression in favor of the alpha-isoform in six out of eight striated muscles (exceptions: soleus muscle and tongue) without consistent relationship to changes in the expression of mitochondrial markers. In summary, our study demonstrated the nNOS alpha-isoform expression to be related to mitochondrial content/activity and to be up-regulated by up-stream PGC-1alpha in striated muscles, particularly in those enriched with type-2 oxidative fibers implying a functional convergence of the two signaling systems in these fibers.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Nitric Oxide - Volume 77, 1 July 2018, Pages 35-43
Journal: Nitric Oxide - Volume 77, 1 July 2018, Pages 35-43
نویسندگان
Oliver Baum, Dea Aaldijk, Anna Lena Engeli, Matthias Spree, Serge Summermatter, Christoph Handschin, Andreas Zakrzewicz,