کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8352613 | 1541891 | 2018 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
AmDREB2C, from Ammopiptanthus mongolicus, enhances abiotic stress tolerance and regulates fatty acid composition in transgenic Arabidopsis
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کلمات کلیدی
ABAAmmopiptanthus mongolicusDREB transcription factorNRDDREBDREhsfNLSCBFHspP5CSPPTGFPCrtAP2/ERF - AP2 / ERFAbiotic stress - استرس بی روحabscisic acid - اسید آبسزیکFatty acid desaturase - اسید لاکتیک اسیدPolyunsaturated fatty acid - اسید چرب غیر اشباعPUFA - اسید چرب چند غیراشباعstandard deviation - انحراف معیارFAD - بدTAD - بلهtranscription activation domain - دامنه فعال سازی رونویسیnuclear localization signal - سیگنال محلی سازی هسته ایC-repeat binding factor - عامل اتصال C تکرارTranscription factor - عامل رونویسیdehydration-responsive element - عنصر واکنش دهنده کم آبیPhosphinothricin - فسفینوتریکینFatty acid methyl ester - متیل استر اسید چربFAME یا fatty acid methyl esters - متیل استرهای اسید چربHeat shock protein - پروتئین شوک حرارتgreen fluorescent protein - پروتئین فلورسنت سبز
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Dehydration-responsive element-binding (DREB) transcription factors (TFs) play a vital role in plant response to abiotic stresses. However, little is known about DREB TFs in plants adapted to harsh environments and in the formation of polyunsaturated fatty acids (PUFAs), a major membrane component closely associated with plant stress tolerance. Here, we characterized AmDREB2C in Ammopiptanthus mongolicus (Maxim. ex kom.) Cheng F., a desert evergreen broadleaf shrub with a high tolerance to harsh environments. AmDREB2C encodes a canonical DREB2-type TF, and the protein was localized in the nucleus. AmDREB2C had the highest expression levels in leaves of naturally growing shrubs in the wild during the winter season of a year of sampling. The expression was also induced by cold, heat and drought stresses in laboratory-cultured seedlings. Moreover, AmDREB2C was most abundantly expressed in young leaves and immature seeds rather than other tissues of the shrubs. Constitutive expression of AmDREB2C in Arabidopsis enhanced freezing, heat and drought tolerances of the transgenic plants, likely through inducing the expression of important stress-responsive genes. The transgene also increased the level of linolenic acid (C18:3), a major PUFA in most plant species, in leaves and seeds of the transgenic plants. Correspondingly, the transcription of FAD3, FAD7 and FAD8, three genes encoding fatty acid desaturases (FADs) responsible for the production of C18:3, showed a differential up-regulation in these two organs. This study provides new insight into the underlying molecular mechanisms of A. mongolicus' ability to endure harsh environments and DREB TF regulation of fatty acid desaturation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Physiology and Biochemistry - Volume 130, September 2018, Pages 517-528
Journal: Plant Physiology and Biochemistry - Volume 130, September 2018, Pages 517-528
نویسندگان
Yumei Yin, Xiaoxu Jiang, Meiyan Ren, Min Xue, Dina Nan, Zhilin Wang, Yanping Xing, Maoyan Wang,