کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8354148 | 1541920 | 2016 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Reference gene selection for quantitative real-time PCR in Solanum lycopersicum L. inoculated with the mycorrhizal fungus Rhizophagus irregularis
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کلمات کلیدی
EF1elongation factor 1 α28S ribosomal RNARibosomal protein L2PGK28S rRNAHprtβ2MqPCR18S rRNAAMFTBPALBGAPDHACTβ-tubulinRNA polymerase II - آرانای پلیمراز II 18S ribosomal RNA - RNA ریبوزوم 18Sβ2-microglobulin - β2-میکروگلوبولینcycle threshold - آستانه چرخهAlbumin - آلبومینactin - اکتین phosphoglycerate kinase - فسفوگلیسرات کینازArbuscular mycorrhizal fungi - قارچ های میکوریزا آربوسکولارhypoxanthine phosphoribosyl transferase - هیپوکسانتین فسفریبوسیل ترانسفرازtub - وانribosomal protein - پروتئین ریبوزومیprotein phosphatase 2A - پروتئین فسفاتاز 2AReference genes - ژنهای مرجعUBI - که در آنglyceraldehyde-3-phosphate dehydrogenase - گلیسرالیدید-3-فسفات دهیدروژنازUbiquitin - یوبیکویتین
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
The gene expression stability of candidate reference genes in the roots and leaves of Solanum lycopersicum inoculated with arbuscular mycorrhizal fungi was investigated. Eight candidate reference genes including elongation factor 1 α (EF1), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), phosphoglycerate kinase (PGK), protein phosphatase 2A (PP2Acs), ribosomal protein L2 (RPL2), β-tubulin (TUB), ubiquitin (UBI) and actin (ACT) were selected, and their expression stability was assessed to determine the most stable internal reference for quantitative PCR normalization in S. lycopersicum inoculated with the arbuscular mycorrhizal fungus Rhizophagus irregularis. The stability of each gene was analysed in leaves and roots together and separated using the geNorm and NormFinder algorithms. Differences were detected between leaves and roots, varying among the best-ranked genes depending on the algorithm used and the tissue analysed. PGK, TUB and EF1 genes showed higher stability in roots, while EF1 and UBI had higher stability in leaves. Statistical algorithms indicated that the GAPDH gene was the least stable under the experimental conditions assayed. Then, we analysed the expression levels of the LePT4 gene, a phosphate transporter whose expression is induced by fungal colonization in host plant roots. No differences were observed when the most stable genes were used as reference genes. However, when GAPDH was used as the reference gene, we observed an overestimation of LePT4 expression. In summary, our results revealed that candidate reference genes present variable stability in S. lycopersicum arbuscular mycorrhizal symbiosis depending on the algorithm and tissue analysed. Thus, reference gene selection is an important issue for obtaining reliable results in gene expression quantification.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Physiology and Biochemistry - Volume 101, April 2016, Pages 124-131
Journal: Plant Physiology and Biochemistry - Volume 101, April 2016, Pages 124-131
نویسندگان
Alejandra Fuentes, Javier Ortiz, Nicolás Saavedra, Luis A. Salazar, Claudio Meneses, Cesar Arriagada,