کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8355024 | 1541948 | 2013 | 15 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Proteomic study of 'Moncada' mandarin buds from on- versus off-crop trees
ترجمه فارسی عنوان
مطالعه پروتئومیک از جوانه های ماندارین مونکادا از درختان بیرونی
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کلمات کلیدی
immobilised pH gradient2D DIGEDTTPMSFQ-TOFIPG - GPALC–MS–MS - LC-MS-MStwo-dimensional difference gel electrophoresis - الکتروفورز ژل تفاوت دو بعدیsodium dodecyl sulphate-polyacrylamide gel electrophoresis - الکتروفورز ژل دوده سولفات سدیم پلی آکریل آمیدSDS-PAGE - الکتروفورز ژل پلی آکریل آمیدAlternate bearing - تحمل جایگزینBuds - جوانهdithiothreitol - دیتیوتریتولquadrupole time-of-flight mass spectrometer - طیف سنج جرمی چهار ساعته پروازphenylmethylsulphonyl fluoride - فنیل متیل سولفونیل فلورایدMALDI-MS - مالدی MSAmino acid metabolism - متابولیسم آمینو اسیدCarbohydrate metabolism - متابولیسم کربوهیدراتCitrus - مرکباتIsoelectric point - نقطه ایزوالکتریکProteome - پروتئومCHAPS - چاپسliquid chromatography coupled with tandem mass spectrometry - کروماتوگرافی مایع همراه با طیف سنجی جرمی دو طرفه
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
چکیده انگلیسی
A proteomic analysis of buds from mandarin trees with contrasting fruit load (on- and off-crop trees) was carried out during the onset of low-temperature induction. The aim of the study was to find out more about the molecular mechanism relating to alternate bearing in Citrus and its relationship with flowering. The 'Moncada' variety (Clementine 'Oroval' x 'Kara' mandarin), displaying remarkable behaviour in alternate production, was used in this study. From 2D DIGE gel, 192 spots were isolated: 97 showed increased expression in the off-crop buds as compared to the on-crop buds, while 95 exhibited enhanced expression in the on-crop buds versus the off-crop buds. These spots were identified by MALDI-MS or LC-MS-MS. The largest groups of proteins up-expressed in the off-crop buds were the proteins involved in carbohydrate and amino acid metabolism, and the proteins expressed in response to stimuli such as reactive oxygen species. The largest groups of proteins up-expressed in the on-crop buds were related to primary metabolism, oxidative stress and defence responses. Depending on their function, some of these proteins can stimulate the flowering, such as fructose-bisphosphate aldolase or leucine-rich repeat transmembrane protein kinase, while others can inhibit it, such as cytochrome c oxidase subunit II. Twenty-two other proteins with unknown functions were up-expressed in the on- or off-crop buds.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Physiology and Biochemistry - Volume 73, December 2013, Pages 41-55
Journal: Plant Physiology and Biochemistry - Volume 73, December 2013, Pages 41-55
نویسندگان
Natalia Muñoz-Fambuena, Carlos Mesejo, Carmina Reig, Manuel AgustÃ, Susana Tárraga, Purificación Lisón, Domingo J. Iglesias, Eduardo Primo-Millo, M. Carmen González-Mas,