کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8357617 | 1542051 | 2015 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Characterization of multiple SPS knockout mutants reveals redundant functions of the four Arabidopsis sucrose phosphate synthase isoforms in plant viability, and strongly indicates that enhanced respiration and accelerated starch turnover can alleviate th
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کلمات کلیدی
6PGDHPEPAGPMDHUDPGIDHG6PPGMG6PDHG3PDHSPSF6PTPTPGIPPaseUGPG1Punit of enzyme activityUDPG pyrophosphorylaseOPPP3-PGATCAADPGSPPSDH3-phosphoglycerate - 3-فسفوگلسرات6-phosphogluconate dehydrogenase - 6-فسفوگلاوکونات دهیدروژنازGenetic redundancy - افزونگی ژنتیکیinorganic orthophosphate - اوراتوفسفات معدنیIsocitrate dehydrogenase - ایزوسیترات دهیدروژنازFunctional interaction - تعامل کاربردیDevelopment - رشدGrowth - رشدDAG - روزdays after germination - روز بعد از جوانه زدنSucrose - ساکارزSucrose-phosphate synthase - سداز فسفات سولفاتsucrose-6-phosphate - سقز -6-فسفاتSucrose synthase - سنتاز ساکاروزSuSy - سوزیsuccinate dehydrogenase - سوکسیناد دهیدروژنازfructose-6-phosphate - فروکتوز 6-فسفاتPhosphoglucose isomerase - فسفو گلوکوز ایزومرازphosphoglucomutase - فسفوگلوکوموتازCarbohydrate metabolism - متابولیسم کربوهیدراتOxidative pentose phosphate pathway - مسیر پوسیدگی فسفات اکسیداتیوmalate dehydrogenase - ملات دهیدروژنازstarch synthase - نشاسته سنتازcontinuous light - نور مداومwild type - نوع وحشیStomatal conductance - هدایت گرمایشیhexokinase - هگزوکینازpyruvate kinase - پیرووات کینازtricarboxylic acid cycle - چرخه اسید تریکاربوکیلیکglucose-1-phosphate - گلوکز 1-فسفاتglucose-6-phosphate - گلوکز 6-فسفاتADP-glucose - گلوکز ADPUDP-glucose - گلوکز UDPglyceraldehyde-3-phosphate dehydrogenase - گلیسرالیدید-3-فسفات دهیدروژناز
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
We characterized multiple knock-out mutants of the four Arabidopsis sucrose phosphate synthase (SPSA1, SPSA2, SPSB and SPSC) isoforms. Despite their reduced SPS activity, spsa1/spsa2, spsa1/spsb, spsa2/spsb, spsa2/spsc, spsb/spsc, spsa1/spsa2/spsb and spsa2/spsb/spsc mutants displayed wild type (WT) vegetative and reproductive morphology, and showed WT photosynthetic capacity and respiration. In contrast, growth of rosettes, flowers and siliques of the spsa1/spsc and spsa1/spsa2/spsc mutants was reduced compared with WT plants. Furthermore, these plants displayed a high dark respiration phenotype. spsa1/spsb/spsc and spsa1/spsa2/spsb/spsc seeds poorly germinated and produced aberrant and sterile plants. Leaves of all viable sps mutants, except spsa1/spsc and spsa1/spsa2/spsc, accumulated WT levels of nonstructural carbohydrates. spsa1/spsc leaves possessed high levels of metabolic intermediates and activities of enzymes of the glycolytic and tricarboxylic acid cycle pathways, and accumulated high levels of metabolic intermediates of the nocturnal starch-to-sucrose conversion process, even under continuous light conditions. Results presented in this work show that SPS is essential for plant viability, reveal redundant functions of the four SPS isoforms in processes that are important for plant growth and nonstructural carbohydrate metabolism, and strongly indicate that accelerated starch turnover and enhanced respiration can alleviate the blockage of sucrose biosynthesis in spsa1/spsc leaves.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Science - Volume 238, September 2015, Pages 135-147
Journal: Plant Science - Volume 238, September 2015, Pages 135-147
نویسندگان
Abdellatif Bahaji, Edurne Baroja-Fernández, Adriana Ricarte-Bermejo, Ángela MarÃa Sánchez-López, Francisco José Muñoz, Jose M. Romero, MarÃa Teresa Ruiz, Marouane Baslam, Goizeder Almagro, MarÃa Teresa Sesma, Javier Pozueta-Romero,