کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8357644 | 1542051 | 2015 | 14 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Functional characterization of FaNIP1;1 gene, a ripening-related and receptacle-specific aquaporin in strawberry fruit
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کلمات کلیدی
NCEDnodulin 26-like intrinsic proteinmajor intrinsic proteinsYNBXIPCFDAMIPSAquaporinsNDGAVWCNIPMurashige & Skoog mediumGFPABA2,4-D - 2،4-D2,4-dichlorophenoxyacetic acid - 2،4-dichlorophenoxyacetic اسید9-cis-epoxycarotenoid dioxygenase - 9-cis-epoxycarotinoid dioxygenasepIPS - PIPSabscisic acid - اسید آبسزیکnordihydroguaiaretic acid - اسید نوردی هیدروگالیارتیActivation energy - انرژی فعال سازیPhotosynthetically active radiation - تابش فعال فتوسنتزیStrawberry - توت فرنگیPar - توسطvolumetric water content - حجم آب حجمیWater transport - حمل و نقل آبDaba - دافاCarboxyfluorescein diacetate - دیافراکت کربوکسیفلوورسسینLuria-Bertani medium - رسانه Luria-BertaniRipening - رطوبتSips - سیر می شودPermeability coefficient - ضریب نفوذپذیریPlasma membrane - غشای پلاسماMoisture content - محتوای رطوبتHIP - مفصل ران یا مفصل هیپFruit - میوهTIPS - نکاتgreen fluorescent protein - پروتئین فلورسنت سبزtonoplast intrinsic proteins - پروتئین های ذاتی تنوپلاستplasma membrane intrinsic proteins - پروتئین های ذاتی غشاء پلاسماHPLC - کروماتوگرافی مایعی کارا
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Strawberry fruit (Fragaria Ã ananassa) is a soft fruit with high water content at ripe stage (more than 90% of its fresh weight). Aquaporins play an important role in plant water homeostasis, through the facilitation of water transport and solutes. We report the role played by FaNIP1;1 in the receptacle ripening process. The analysis by qRT-PCR of FaNIP1;1 showed that this gene is mainly expressed in fruit receptacle and has a ripening-related expression pattern that was accompanied by an increase in both the abscisic acid and water content of the receptacle throughout fruit ripening. Moreover, FaNIP1;1 was induced in situations of water deficit. Additionally, we show that FaNIP1;1 expression was positively regulated by abscisic acid and negatively regulated by auxins. The water transport capacity of FaNIP1;1 was determined by a stopped-flow spectroscopy in yeast over-expressing FaNIP1;1. Glycerol, H2O2 and boron transport were also demonstrated in yeast. On the other hand, GFP-FaNIP1;1 fusion protein was located in plasma membrane. In conclusion, FaNIP1;1 seems to play an important role increasing the plasma membrane permeability, that allows the water accumulation in the strawberry fruit receptacle throughout the ripening process.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Science - Volume 238, September 2015, Pages 198-211
Journal: Plant Science - Volume 238, September 2015, Pages 198-211
نویسندگان
Francisco J. Molina-Hidalgo, Laura Medina-Puche, Samuel Gelis, José Ramos, Farzana Sabir, Graça Soveral, Catarina Prista, Raquel Iglesias-Fernández, José L. Caballero, Juan Muñoz-Blanco, Rosario Blanco-Portales,