کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8404052 | 1544688 | 2018 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Proteomic analyses of ram (Ovis aries) testis during different developmental stages
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کلمات کلیدی
HSPB1TFATPM3ApoA-ICA2TPI1RBP4CFL1Ribosomal protein S12GSTPHeat shock protein beta-1MALDI TOF-MSRPS12ddH2OGLO1STMN1Glutathione S-transferase PTXNMALDI-TOF/TOF-MSFTH1PGAM1qRT-PCRCarbonic anhydrase 2PVDFNCBIPRDX6PBSACN2-DEDABTCAPARK7PBSTPLECPMFsodium dodecyl sulfate – polyacrylamide gel electrophoresisIPG - GPAimmobilized pH gradient - gradient pH immobilizedMS/MS - MS / MSApolipoprotein A-I - آپولیپوپروتئین A-IPeptide mass fingerprint - اثر انگشت پپتیدهStathmin - استاتمینیAcetonitrile - استونیتریلTrifluoroacetic acid - اسید Trifluoroacetictrichloroacetic acid - اسید ترشکلراکتیکtwo dimensional electrophoresis - الکتروفورز دو بعدیSDS-PAGE - الکتروفورز ژل پلی آکریل آمیدbeta-actin - بتا آکتینTestis - بیضهANOVA - تحلیل واریانس Analysis of varianceone-way analysis of variance - تحلیل واریانس یک راههTUBB - توبthioredoxin - تیرودوکسینRoom temperature - دمای اتاقdiaminobenzidine - دیامینو بنزیدینpolyvinyl difluoride - دیوفوردین پلیوینیلRam - رمquantitative real time PCR - زمان واقعی PCR کمیMass spectrometry - طیف سنجی جرمیTandem mass spectrometry - طیف سنجی جرمی پشت سر هم یا متوالیActb - عملPhosphoglycerate mutase 1 - فسفوگلسرات ماتاز 1phosphate buffer solution - محلول بافر فسفاتNational Center for Biotechnology Information - مرکز ملی اطلاعات بیوتکنولوژیGene ontology - هستیشناسی ژنیProteomics - پروتئومیکسRetinol-binding protein 4 - پروتئین اتصال دهنده رتینول 4cofilin-1 - کوفیلین 1
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Male reproductive capacity is essential for animal breeding and reproduction. In males, the testes produce sperm and secrete androgen, processes which require precise regulation by multiple proteins. The composition of proteins in the ram testes has not yet been studied systematically, thus, the application of proteomics to explore differential protein regulation during ram testes development is of great significance. In the present study, ram testes were studied at five different developmental phases to assess postnatal differences in protein regulation. Two dimensional electrophoresis (2-DE) was used to separate ram testes proteins at each developmental phase, yielding 45 different proteins, 37 of which were identified by Matrix Assisted Laser Desorption Ionization-Time of Flight-Time of Flight-Mass Spectrometry (MALDI-TOF/TOF-MS). Gene Ontology (GO) was used to specifically annotate the biological process, cellular composition, and molecular function of each identified protein. Most of the identified proteins were involved in structural formation, development, reproduction, and apoptosis of the testicular spermatogenic tissue and spermatozoa. Quantitative real time PCR (qRT-PCR), western blot and immunohistochemical methods were used to verify the proteins, and the results were consistent with that of 2-DE. The proteins that were different in abundance that were identified in this study can be used as biomarkers in future studies of ram reproduction.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Animal Reproduction Science - Volume 189, February 2018, Pages 93-102
Journal: Animal Reproduction Science - Volume 189, February 2018, Pages 93-102
نویسندگان
Zengkui Lu, Youji Ma, Quanwei Zhang, Xingxu Zhao, Yong Zhang, Liping Zhang,