Development of sandwich ELISA for detection and quantification of human and murine a disintegrin and metalloproteinase17

► ADAM17 implicates in many physiological processes. ► Using hybridoma technology, we generated three monoclonal antibodies against ADAM17. ► The specificity of the antibodies was test by FACS, ELISA and WB. ► We developed sandwich ELISA to detect and quantify human and murine ADAM17.