کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8420127 | 1545772 | 2008 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Development of a new antibody to the human inhibin/activin βB subunit and its application to improved inhibin B ELISAs
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
HRPInhibin BTMBHbRFCSDSLNIBSCPMSOBIPBSSDSActivin Bsodium dodecyl sulfate-polyacrylamide gel electrophoresis - الکتروفورز ژل دوده سولفات سدیم پلی آکریل آمیدSDS-PAGE - الکتروفورز ژل پلی آکریل آمیدtetramethylbenzidine - تترامتیل بنزیدینEnzyme-linked immunosorbent assay - تست الیزاELISA - تست الیزاsodium dodecyl sulfate - سدیم دودسیل سولفاتfoetal calf serum - سرم گوساله جنینPhosphate buffered saline - فسفات بافر شورNational Institute for Biological Standards and Control - مؤسسه ملی استاندارد و کنترل زیست شناسیfollicle stimulating hormone - هورمون تحریک کننده فولیکولFSH - هورمون محرکه فولیکولی Immunoassay - واکنش های ایمونواسیMonoclonal antibodies - پادتنهای تَکتیرهHorseradish peroxidase - پراکسیداز هوررادیشpolyethylene glycol - پلی اتیلن گلیکولPEG - پلیاتیلن گلیکول high-performance liquid chromatography - کروماتوگرافی مایعی کاراHPLC - کروماتوگرافی مایعی کارا
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوتکنولوژی یا زیستفناوری
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Development of a new antibody to the human inhibin/activin βB subunit and its application to improved inhibin B ELISAs Development of a new antibody to the human inhibin/activin βB subunit and its application to improved inhibin B ELISAs](/preview/png/8420127.png)
چکیده انگلیسی
Inhibin B has emerged as a clinically useful analyte for studies of reproductive function in both men and women. The antibody to the βB subunit (C5) used in current commercially available assays (DSL and OBI) was raised in this laboratory to a synthetic peptide from the βB subunit. These assays require pre-treatment of samples with hydrogen peroxide to oxidise two methionines in the epitope to the sulfoxide for full immunoreactivity. It was also claimed that this antibody cross-reacted significantly with inhibin A leading to a 0.5% cross-reaction of inhibin A in the current generation of immunoassays. Both of the above immunoassays required overnight incubation with sample. The development of improved antibodies to the βB subunit has proved difficult due to the conservation of the βB subunit between species. We describe the development of new monoclonal antibodies to the βB subunit, by immunisation of mice with recombinant X. laevis activin B using the RIMMS method of immunisation. The result has been the development of highly specific antibodies in a short time period. One of these antibodies 46A/F is shown to be a highly effective capture antibody in a human inhibin B ELISA, without any sample pre-treatment. The results of the validation of an improved inhibin B assay using 46A/F as the capture antibody are shown, with comparison to one of the commercially available inhibin B assays. Overall, the inhibin B assay is simplified and the performance improved by using this new antibody 46A/F. It was further shown that the cross-reaction of inhibin A in both the OBI and DSL inhibin B ELISAs is ten fold less than previously reported. This can be attributed to the poor quality of recombinant inhibin B available for use as standard in 1996. Although the present generation of inhibin B assays has proved adequate to enable the physiological function of inhibin to be determined and novel clinical applications found, the simplification of the assay made possible by the improved antibody should make possible a new generation of more rapid, sensitive, convenient and robust tools for routine use.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Immunological Methods - Volume 329, Issues 1â2, 1 January 2008, Pages 102-111
Journal: Journal of Immunological Methods - Volume 329, Issues 1â2, 1 January 2008, Pages 102-111
نویسندگان
Helen Ludlow, Shanthi Muttukrishna, Marko Hyvönen, Nigel P. Groome,