کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8451760 | 1547696 | 2018 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Subpopulations of cancer stem cells found in papillary thyroid carcinoma
ترجمه فارسی عنوان
زیرمجموعه سلول های بنیادی سرطانی در کارسینوم پاپیلر تیروئید یافت می شود
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
ALDHCSCNEAAEGFPTCPBSFACSbFGFDMEMaldehyde dehydrogenase - آلدئید دهیدروژنازnonessential amino acids - اسیدهای غیر ضروریDulbecco's modification of Eagle's medium - اصلاح Dulbecco از محیط عقابLDA - تخصیص پنهان دیریکلهfluorescence-activated cell sorting - دسته بندی سلول های فعال فلورسنسPrimary Cells - سلولهای اولیهcancer stem cell - سلولهای بنیادی سرطانیCancer stem cells - سلولهای بنیادی سرطانیepidermal growth factor - عامل رشد اپیدرمیbasic fibroblast growth factor - فاکتور رشد فیبروبلاست پایهPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریThyroid carcinoma - کارسینوم تیروئیدPapillary thyroid carcinoma - کارسینوم پاپیلاری تیروئید
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
چکیده انگلیسی
Papillary thyroid carcinoma (PTC) is the most common form of thyroid cancer and while it has a generally good prognosis, tumor recurrence remains a major clinical challenge. Studying laboratory cell lines as well as clinical specimens indicate that PTC may follow the cancer stem cell (CSC) model. However, CSC characteristics relevant in PTC initiation and progression remain largely unknown. Here we studied a population of sphere-growing tumor cells isolated from primary cultures of clinical PTC. These sphere-growing cells consisted of aldehyde dehydrogenase positive (ALDH+) and ALDH negative (ALDH-) cell subpopulations and demonstrated a hierarchical pattern of cell division. Using combinations of selective depletion, specific inhibition and cell sorting, we found that both subpopulations of the sphere cells were able to self-renew and initiate xenograft tumors independently, and fulfilled the definition of CSC. Importantly, when the subpopulations functioned together, the cancer-initiation efficiency and the xenograft tumor progression were significantly enhanced compared to either subpopulation alone. These data revealed crucial roles of ALDH- CSC in PTC biology and suggested that CSC subpopulations function cooperatively to control PTC initiation and progression. Together, our study indicates that CSC subpopulations isolated from clinical specimens offer unprecedented opportunities for investigating PTC pathogenesis and developing effective therapies.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Cell Research - Volume 362, Issue 2, 15 January 2018, Pages 515-524
Journal: Experimental Cell Research - Volume 362, Issue 2, 15 January 2018, Pages 515-524
نویسندگان
Saied Mirshahidi, Alfred Simental, Steve C. Lee, Pedro A. De Andrade Filho, Nathaniel R. Peterson, Wenlong Cao, Rosalia de Necochea-Campion, Hao Yang, Penelope Duerksen-Hughes, Xiangpeng Yuan,