کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8461287 | 1549007 | 2013 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Regional regulation of glutamate signaling during cuprizone-induced demyelination in the brain
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کلمات کلیدی
SLCHprtPLPmGluRscuprizoneEAATN-methyl-d-aspartateNMDAGLASTEAEECLCorpus callosumAMPAGRMIBAHRPMBPTGF-β1SDSMOGPBSPVDFGFAPBSA - BSAdemyelination - demyelinimasGLT - GrlatMAPK - MAPKProteolipid protein - Proteolipid پروتئینbovine serum albumin - آلبومین سرم گاوexperimental allergic encephalomyelitis - آنسفالومیلیت آلرژیک تجربیα-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid - اسید α-آمینو 3-هیدروکسی-5-متیل-4-ایزوکسول پپونیکImmunohistochemistry - ایمونوهیستوشیمیIHC - ایمونوهیستوشیمیenhanced chemiluminescence - بهبود شیمیایی لومنTransforming growth factor-beta 1 - تبدیل فاکتور رشد بتا 1solute carrier - حامل رقیبGlutamate transporter - حمل و نقل گلوتاماتexcitatory amino acid transporter - حمل کننده آمینو اسید هیجان انگیزCNS - دستگاه عصبی مرکزیsodium dodecylsulfate - سدیم دودسیل سولفاتcentral nervous system - سیستم عصبی مرکزیPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریMultiple sclerosis - مولتیپل اسکلروزیس(ام اس)Nitric oxide - نیتریک اکسیدhypoxanthine-guanine phosphoribosyltransferase - هیپوکسانتین-گوانین فسفریبوسیل ترانسفرازHorseradish peroxidase - پراکسیداز هوررادیشGlial fibrillary acidic protein - پروتئین اسیدی فیبریلاسیون گلایالMyelin basic protein - پروتئین پایه میلینmitogen-activated protein kinase - پروتئین کیناز فعال با mitogenmyelin oligodendrocyte glycoprotein - گلیکوپروتئین الیگودندروسیت میلینMetabotropic glutamate receptors - گیرنده های متابوتروپیک گلوتاماتglutamate receptor, metabotropic - گیرنده گلوتامات، متابوتروپیکGlutamate receptor - گیرنده گلومات
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Glutamate excitotoxicity is associated with a wide range of neurodegenerative disorders and also seems to be involved in the pathology of demyelinating disorders such as multiple sclerosis (MS). Cuprizone-induced toxic demyelination shows clear characteristics of MS such as demyelination and axonal damage without the involvement of the innate immune system. In this study, we have evaluated glutamate signaling during cuprizone-induced demyelination in the white and gray matter of mouse brain by studying the expression of ionotropic and metabotropic glutamate-receptors and -transporters by Affymetrix gene array analysis, followed by real-time PCR and western blot analysis. Cellular localization of glutamate transporters was investigated by fluorescence double-labeling experiments. Comparing white and gray matter areas, the expression of glutamate receptors was region-specific. Among NMDA receptor subunits, NR2A was up-regulated in the demyelinated corpus callosum (CC), whereas the metabotropic glutamate receptor mGluR2 was down-regulated in demyelinated gray matter. Glutamate-aspartate transporter (GLAST) co-localizing with GFAP+ astrocytes was increased in both demyelinated CC and telencephalic cortex, whereas Slc1a4 transporter was up-regulated only in CC. Our data indicate that cuprizone treatment affects glutamate-receptors and -transporters differently in gray and white matter brain areas revealing particularly regulation of GLAST and Slc1a4 compared with other genes. This might have an important influence on brain-region selective sensitivity to neurotoxic compounds and the progression of demyelination as has been reported for MS and other demyelinating neurological diseases.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Annals of Anatomy - Anatomischer Anzeiger - Volume 195, Issue 5, October 2013, Pages 415-423
Journal: Annals of Anatomy - Anatomischer Anzeiger - Volume 195, Issue 5, October 2013, Pages 415-423
نویسندگان
Abolfazl Azami Tameh, Tim Clarner, Cordian Beyer, Mohammad Ali Atlasi, Gholamreza Hassanzadeh, Homayoun Naderian,