کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8474956 | 1550437 | 2014 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Freshly isolated mitochondria from failing human hearts exhibit preserved respiratory function
ترجمه فارسی عنوان
میتوکندری تازه از هم گسیختگی قلب انسان، تظاهرات تنفسی را حفظ می کند
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کلمات کلیدی
acyl-CoA dehydrogenase, very long chainNICMHFrEFCPT2PGC1αIFMAMPKPDHBACADMPPIALVIDdACADVLPDK4ICMP/E ratioGLUT4GLUT1flavin adenine dinucleotidePeptidylprolyl isomerase A (cyclophilin A)me3RT-PCRSSMCD36ACADLPPARαFCCPRCRSucc5′ AMP-activated protein kinase - 5 'پروتئین کیناز فعال AMPNon-Ischemic Cardiomyopathy - Cardiomyopathy غیر ایسکمیNAD+ - NAD +left ventricle - بطن چپHADHA - حدیثglucose transporter 4 - حمل کننده گلوکز 4cluster of differentiation 36 - خوشه تمایز 36electron transport chain - زنجیره انتقال الکترونSuccinate - سوکینتینHuman heart failure - ضربان قلب انسانیMitochondrial function - عملکرد میتوکندریOxidative phosphorylation - فسفوریلاسیون اکسیداتیوsubsarcolemmal mitochondria - میتوکندری subarcolemmalinterfibrillar mitochondria - میتوکندری بین فیبریلاسیونheart failure - نارسایی قلبیheart failure with reduced ejection fraction - نارسایی قلبی با کاهش کسر تخلیهrespiratory control ratio - نسبت کنترل تنفسیnicotinamide adenine dinucleotide - نیکوتین آمید adenine dinucleotideETc - و غیرهPulmonary hypertension - پرفشاری خون ریویPhtN - پی اچ پیPyruvate dehydrogenase kinase 4 - پیرووات دهیدروژناز کیناز 4carnitine palmitoyltransferase II - کاردینیتین پالمیتویل ترانسفراز IIischemic cardiomyopathy - کاردیومیوپاتی ایسکمیcarbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone - کربونیل سیانید 4- (trifluoromethoxy) phenylhydrazoneglucose transporter 1 - گلوکز 1peroxisome proliferator-activated receptor α - گیرنده پروتئینی فعال پروکسایزوم α
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
چکیده انگلیسی
In heart failure mitochondrial dysfunction is thought to be responsible for energy depletion and contractile dysfunction. The difficulties in procuring fresh left ventricular (LV) myocardium from humans for assessment of mitochondrial function have resulted in the reliance on surrogate markers of mitochondrial function and limited our understanding of cardiac energetics. We isolated mitochondria from fresh LV wall tissue of patients with heart failure and reduced systolic function undergoing heart transplant or left ventricular assist device placement, and compared their function to mitochondria isolated from the non-failing LV (NFLV) wall tissue with normal systolic function from patients with pulmonary hypertension undergoing heart-lung transplant. We performed detailed mitochondrial functional analyses using 4 substrates: glutamate-malate (GM), pyruvate-malate (PM) palmitoyl carnitine-malate (PC) and succinate. NFLV mitochondria showed preserved respiratory control ratios and electron chain integrity with only few differences for the 4 substrates. In contrast, HF mitochondria had greater respiration with GM, PM and PC substrates and higher electron chain capacity for PM than for PC. Surprisingly, HF mitochondria had greater respiratory control ratios and lower ADP-independent state 4 rates than NFLV mitochondria for GM, PM and PC substrates demonstrating that HF mitochondria are capable of coupled respiration ex vivo. Gene expression studies revealed decreased expression of key genes in pathways for oxidation of both fatty acids and glucose. Our results suggest that mitochondria from the failing LV myocardium are capable of tightly coupled respiration when isolated and supplied with ample substrates. Thus energy starvation in the failing heart may be the result of dysregulation of metabolic pathways, impaired substrate supply or reduced mitochondrial number but not the result of reduced mitochondrial electron transport capacity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Molecular and Cellular Cardiology - Volume 68, March 2014, Pages 98-105
Journal: Journal of Molecular and Cellular Cardiology - Volume 68, March 2014, Pages 98-105
نویسندگان
Andrea M. Cordero-Reyes, Anisha A. Gupte, Keith A. Youker, Matthias Loebe, Willa A. Hsueh, Guillermo Torre-Amione, Heinrich Taegtmeyer, Dale J. Hamilton,