کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8492975 | 1552792 | 2018 | 36 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
In vivo anesthetic effect and mechanism of action of active compounds from Alpinia galanga oil on Cyprinus carpio (koi carp)
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کلمات کلیدی
BZDPAMSSCAMSDNMDAN-methyl-d-aspartate1,8-Cineole - 1،8-CineoleSCb - SCBγ-aminobutyric acid type A - γ-آمینوآبتیریک اسید نوع AMass selective detector - آشکارساز انتخابی توده ایBinding energy - انرژی پیوندbenzodiazepine - بنزودیازپینInhibitory constant - ثابت ماندهcombination index - شاخص ترکیبیMethyl eugenol - متیل اگنولPositive allosteric modulators - مدولاتور آلوزیستریک مثبتAGO - پیشComputational docking - پیوند محاسباتیGABAA - گابا
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم آبزیان
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
In the present study, three active compounds of Alpinia galanga oil (AGO); 1,8-cineole (37.43%), 4-allylphenyl acetate (25.97%), and methyl eugenol (5.07%) were investigated for anesthetic effect on Cyprinus carpio (koi carp) and their mechanism of action. The anesthetic effect was evaluated by determining the induction time that the fish needed to reach the surgical stage of anesthesia and subsequent recovery time. Among the three test compounds, methyl eugenol showed the shortest induction time and longest recovery time. The induction times of 4-allylphenyl acetate were longer and the recovery times were shorter than 1,8- cineole. For the mechanism of action, the compounds were investigated by computational docking into the benzodiazepine (BZD) site of the γ-aminobutyric acid type A (GABAA) receptor complex model. Ligand-protein docking interaction, i.e., binding energy (ÎGbind) and inhibitory constant (Ki), were calculated. The result of the computational prediction was related to the experimental anesthetic activity, where methyl eugenol showed the lowest ÎGbind of â6.10â¯kcalâ¯molâ1 and Ki of 33.84â¯Î¼M, followed by 4-allylphenyl acetate (with ÎGbind of â5.67â¯kcalâ¯molâ1 and Ki of 70.30â¯Î¼M), and 1,8-cineole (with ÎGbind of â5.39â¯kcalâ¯molâ1 and Ki of 112.40â¯Î¼M). To this end, a comparison of recovery time of the anesthetized fish using flumazenil, a BZD antagonist, was carried out. The fish exposed to all tested compounds resulted a faster recovery in the water tank with flumazenil than that without flumazenil, confirming the receptor binding site of these three compounds are GABAA receptor interaction. It is concluded that the anesthetic effect of AGO on koi carp is according to the three main active compounds; 1,8-cineole, 4-allylphenyl acetate, and methyl eugenol. Moreover, the mechanism of anesthetic action of these compounds is exerting GABAA receptor interaction via the BZD-binding site.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Aquaculture - Volume 496, 1 November 2018, Pages 176-184
Journal: Aquaculture - Volume 496, 1 November 2018, Pages 176-184
نویسندگان
Nattakanwadee Khumpirapang, Siripat Chaichit, Supat Jiranusornkul, Surachai Pikulkaew, Anette Müllertz, Siriporn Okonogi,