کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8507038 | 1555959 | 2018 | 36 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Establishment of a multi-acaricide resistant reference tick strain (IVRI-V) of Rhipicephalus microplus
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Establishment of a multi-acaricide resistant reference tick strain (IVRI-V) of Rhipicephalus microplus Establishment of a multi-acaricide resistant reference tick strain (IVRI-V) of Rhipicephalus microplus](/preview/png/8507038.png)
چکیده انگلیسی
Tick-borne diseases is a global threat and tick resistance to commonly used acaricides is a growing problem, thus calling for improved resistance monitoring tools. To aid in monitoring of resistance in field tick populations, a resistant colony of Rhipicephalus microplus was characterized with the aim to establish a reference multi-acaricide resistant tick strain. Using a standardized adult immersion test, the Lethal Concentration(LC)50 values for deltamethrin, cypermethrin, fenvalerate and diazinon against the laboratory selected resistant tick (LSRT) strain were determined as 306.7â¯ppm, 2776.9â¯ppm, 30262.1â¯ppm and 9458.7â¯ppm. Relative to the susceptible IVRI-I tick strain, the LSRT strain showed 4.78- and 5.84-fold increases in activity of esterases, a 6-fold increase for monooxygenases and a 2.24 fold increase for glutathione S-transferase. In the acetylcholinesterase 2 gene, 22 single nucleotide polymorphisms (SNPs) were identified in the LSRT strain. Four of these SNPs lead to amino acid substitutions and were consistently found in resistant field populations in India. A C190A mutation in the domain II S4-5 linker region of sodium channel gene resulting in a L64I amino acid substitution was recorded in the LSRT strain. Monitorable indicators for the maintenance of the strain, designated as the reference IVRI-V tick strain and representing the first established multi-acaricide resistant tick strain in India, were identified.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Ticks and Tick-borne Diseases - Volume 9, Issue 5, July 2018, Pages 1184-1191
Journal: Ticks and Tick-borne Diseases - Volume 9, Issue 5, July 2018, Pages 1184-1191
نویسندگان
Ashutosh Fular, Anil Kumar Sharma, Sachin Kumar, Gaurav Nagar, Gajanan Chigure, D.D. Ray, Srikant Ghosh,