Biosynthesis of antibiotic chuangxinmycin from Actinoplanes tsinanensis

In this study, the biosynthetic gene cluster of chuangxinmycin (1) was firstly identified, cloned and heterologously expressed and a plausible pathway for its biosynthesis was proposed: L-tryptophan was converted to seco-chuangxinmycin (3) through a series of biochemical reaction processes and by hijacking the sulfur transfer system from primary metabolism using the cluster-situated sulfur carrier protein CxnE. Then the second C-S bond formation was achieved by the cytochrome P450 CxnD.105