کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8514068 | 1556503 | 2017 | 35 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Identification of Excipients for Stabilizing Fiberless Adenovirus as Biopharmaceuticals
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کلمات کلیدی
DLSadenovirus serotype 5Ad5EPDSLSTrpAdenoviral vector - بردار adenoviralViral vectors - بردارهای ویروسیTryptophan - تریپتوفانStability - ثباتbiopharmaceuticals characterization - خصوصیات بیوفارمینتیکTransition temperature - دمای انتقالPhase diagram - دیاگرامهای فازی circular dichroism - رنگ تابی دورانیExcipients - عضلاتCAR - ماشینempirical phase diagram - نمودار فاز تجربیstatic light scattering - پراکندگی نور استاتیکDynamic Light Scattering - پراکندگی نور دینامیکی
موضوعات مرتبط
علوم پزشکی و سلامت
داروسازی، سم شناسی و علوم دارویی
اکتشاف دارویی
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چکیده انگلیسی
Reducing the promiscuous tropism of native adenovirus by using fiberless adenovirus is advantageous toward its use as a gene therapy vector or vaccine component. The removal of the fiber protein on native adenovirus abrogates several undesirable interactions; however, this approach decreases the particle's physical stability. To create stable fiberless adenovirus for pharmaceutical use, the effects of temperature and pH on the particle's stability profile must be addressed. Our results indicate that the stability of fiberless adenovirus is increased when it is stored in mildly acidic conditions around pH 6. The stability of fiberless adenovirus can be further enhanced by using excipients. Excipient screening results indicate that the nonionic surfactant Pluronic F-68 and the amino acid glycine are potential stabilizers because of their ability to increase the thermal transition temperature of the virus particle and promote retention of biological activity after exposure to prolonged thermal stress. Our data indicate that the instability of fiberless adenovirus can be ameliorated by storing the virus in the appropriate environment, and it should be possible to further optimize the virus so that it can be used as a biopharmaceutical.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical Sciences - Volume 106, Issue 7, July 2017, Pages 1764-1771
Journal: Journal of Pharmaceutical Sciences - Volume 106, Issue 7, July 2017, Pages 1764-1771
نویسندگان
Grit Kupgan, Shyamal P. Choudhari, Nicholas H. Flynn, Adane Nigatu, Sravanthi Vupputuri, Wendy L. Picking, William D. Picking, Joshua D. Ramsey,