کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8521079 | 1557717 | 2018 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Sensitive and rapid determination of amantadine without derivatization in human plasma by LC-MS/MS for a bioequivalence study
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کلمات کلیدی
موضوعات مرتبط
علوم پزشکی و سلامت
داروسازی، سم شناسی و علوم دارویی
علوم دارویی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Sensitive and rapid determination of amantadine without derivatization in human plasma by LC-MS/MS for a bioequivalence study Sensitive and rapid determination of amantadine without derivatization in human plasma by LC-MS/MS for a bioequivalence study](/preview/png/8521079.png)
چکیده انگلیسی
A highly sensitive, rapid and rugged liquid chromatography-tandem mass spectrometry (LC-ESI-MS/MS) method was developed for reliable estimation of amantadine (AMD), an antiviral drug in human plasma. The analyte and internal standard (IS), amantadine-d6 (AMD-d6), were extracted from 200â¯ÂµL plasma by solid phase extraction on Phenomenex Strata-X-C 33â¯Âµ cartridges. Chromatography was performed on Synergi⢠Hydro-RP C18 (150â¯mm à 4.6â¯mm, 4â¯Âµm) analytical column using a mixture of acetonitrile and 10â¯mM ammonium formate, pH 3.0 (80:20, v/v) as the mobile phase. Detection and quantitation was done by multiple reaction monitoring in the positive ionization mode for AMD (m/z 152.1 â 135.1) and IS (m/z 158.0 â 141.1) on a triple quadrupole mass spectrometer. The assay was linear in the concentration range of 0.50-500â¯ng/mL with correlation coefficient (r2) ⥠0.9969. The limit of detection of the method was 0.18â¯ng/mL. The intra-batch and inter-batch precisions were ⤠5.42% and the accuracy varied from 98.47% to 105.72%. The extraction recovery of amantadine was precise and quantitative in the range of 97.89%-100.28%. IS-normalized matrix factors for amantadine varied from 0.981 to 1.012. The stability of AMD in whole blood and plasma was evaluated under different conditions. The developed method was successfully applied for a bioequivalence study with 100â¯mg of AMD in 32 healthy volunteers. The reproducibility of the assay was determined by reanalysis of 134 subject samples.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical Analysis - Volume 8, Issue 3, June 2018, Pages 202-207
Journal: Journal of Pharmaceutical Analysis - Volume 8, Issue 3, June 2018, Pages 202-207
نویسندگان
Abhaysingh Bhadoriya, Shivprakash Rathnam, Bhavesh Dasandi, Dharmesh Parmar, Mallika Sanyal, Pranav S. Shrivastav,