کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8524987 | 1557939 | 2018 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Avicularin reversed multidrug-resistance in human gastric cancer through enhancing Bax and BOK expressions
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کلمات کلیدی
5-FU3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromideAvicularinDDPPARPBCAMcl-1H&E - H & EMTT - MTTBax - باکسbicinchoninic acid - بیسینکنینیک اسیدTUNEL - تونلApoptosis - خزان یاختهایGastric cancer - سرطان معدهcisplatin - سیس پلاتین5-fluorouracil - فلوروراسیل-۵، فلوئورواوراسیلMyeloid cell leukemia 1 - لوسمی سلول میلوئید 1Hematoxylin and Eosin - هماتوکسیلین و ائوزینpoly (ADP-ribose) polymerase - پلی (ADP-ribose) پلیمرازBok - کتاب
موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
تومور شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
5-Fluorouracil (5-Fu) and cisplatin (DDP) as important therapies in treatment of human gastric cancer have been widely determined. However, the therapeutic effects are usually hampered due to drug resistance or toxicity at high concentrations for application. Avicularin (AL, quercetin-3-α-l-arabinofuranoside), a bio-active flavonol isolated from a number of plants, has been reported to display diverse pharmacological properties. In this study, we explored the hypothesis by which AL reversed 5-Fu or DDP resistance in gastric cancer and the underlying molecular mechanism. Here, in vitro, the drug-resistant cancer cells were incubated to AL or DDP alone or the combination of AL and DDP. Then, MTT, colony formation, Hoechst 33258, flow cytometry and western blot analysis were used to investigate the effects of AL in the regulation of drug-resistance gastric cancer cells. The results indicated that AL treatment markedly re-sensitizes the drug resistant cells (SGC-7901/5-Fu and SGC-7901/DDP) to cytotoxicity of 5-Fu or DDP. Molecular mechanism analysis indicated that AL and DDP combination treatment enhanced apoptosis in SGC-7901/DDP cells, accompanied with the up-regulation of cleaved Caspase-3 and PARP, as well as the activation of pro-apoptotic signals, including Bax and BOK. Significantly, down regulation of Bax or BOK expressions using Bax siRNA or BOK siRNA decreased the inhibitory role of DDP in apoptosis of SGC-7901/DDP cells pretreated with AL, demonstrating that AL-reversed DDP resistance was associated with Bax and BOK expression. In vivo, AL and DDP combination significantly reduced gastric tumor growth. Immunohistochemical analysis indicated that co-treatment of AL and DDP significantly induced apoptosis, and reduced tumor cell proliferation in tumor tissue samples. Furthermore, we also found that the Bax, BOK, cleaved Caspase-3 and PARP expression in tumor tissues were highly induced by AL and DDP co-treatment. Together, our findings may provide a novel combination therapeutic strategy in treatment of human gastric cancer.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomedicine & Pharmacotherapy - Volume 103, July 2018, Pages 67-74
Journal: Biomedicine & Pharmacotherapy - Volume 103, July 2018, Pages 67-74
نویسندگان
Xiang-Feng Guo, Ji-Peng Liu, Si-Quan Ma, Peng Zhang, Wen-De Sun,