کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8526170 | 1557941 | 2018 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Helix aspersa maxima mucus exhibits antimelanogenic and antitumoral effects against melanoma cells
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کلمات کلیدی
MMPMBSPBSBCAIBMXDMEMSDSFBSECM3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide - 3- (4،5-dimethylthiazol-2-yl) -2،5-difenyl tetrazolium bromide3-isobutyl-1-methylxanthine - 3-ایزوبوتیل-1-متیلکسانتینBSA - BSAl-3,4-dihydroxyphenylalanine - L-3،4-دی هیدروکسی فنیل آلانینl-DOPA - L-DOPAMTT - MTTbovine serum albumin - آلبومین سرم گاوbicinchoninic acid - بیسینکنینیک اسیدsodium dodecyl sulfate - سدیم دودسیل سولفاتfetal bovine serum - سرم جنین گاوPhosphate buffered saline - فسفات بافر شورFibronectin - فیبرونکتینFibrinogen - فیبرینوژنExtracellular matrix - ماتریکس خارج سلولیmatrix metalloproteinase - ماتریکس متالوپروتئینازDulbecco’s modified eagle’s medium - محیط عقاب اصلاح شده DulbeccoPoly-l-lysine - پلی-لیزین
موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
تومور شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Snail secretion is currently revolutionizing the world of cosmetics and human skin care. The efficacy of snail secretion in wounds healing has been proven both in vitro and by clinical studies. However, the potential anti-tumor effect of snail secretion was poorly investigated. In this report, our in vitro study showed that Helix aspersa maxima species snail slime (SS) could not only treat melanogenesis but also endowed with anti-tumoral activity against human melanoma cells. Indeed, SS reduced melanin content and tyrosinase activity on B16F10 cells with IC50 values of 288â¯Î¼g/mL and 286â¯Î¼g/mL, respectively, without altering cell viability. This effect was also observed, at a lesser extent, on human melanoma IGR-39 and SK-MEL-28 cell lines. On another hand, SS specifically inhibited the viability of IGR-39 and SK-MEL-28 cells associated to an apoptotic effect highlighted by PARP cleavage. It is worth to note that SS did not affect the viability of B16F10 cells and non tumorigenic HaCaT cells. Interestingly, this extract was found to inhibit migration and invasion of both human melanoma cells through reducing the expression of Matrix metalloproteinase MMP2. Snail slime also exerted a high inhibitory effect on IGR-39 cell adhesion through blocking the function of α2β1 (45%), αvβ3 (38%) integrins and by reducing the expression levels of αv and β1 integrins. The presented results shed light on the potential anti-melanoma effect of SS and support its use against skin diseases.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomedicine & Pharmacotherapy - Volume 101, May 2018, Pages 871-880
Journal: Biomedicine & Pharmacotherapy - Volume 101, May 2018, Pages 871-880
نویسندگان
Chedli Ellijimi, Manel Ben Hammouda, Houcemeddine Othman, Wassim Moslah, Jed Jebali, Hazem Ben Mabrouk, Maram Morjen, Meriam Haoues, José Luis, Naziha Marrakchi, Khadija Essafi-Benkhadir, Najet Srairi-Abid,