کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8544475 | 1561549 | 2018 | 33 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Flubendazole and mebendazole impair migration and epithelial to mesenchymal transition in oral cell lines
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کلمات کلیدی
FAKRac1GEF-H1TGF-βras homolog family member Aepithelial to mesenchymal transition - اپیتلیال به انتقال مزانشیمالBenzimidazoles - بنزیمیدازولTransforming growth factor β - تبدیل فاکتور رشد βEMT - تکنسین فوریتهای پزشکیcell index - شاخص سلولGingival fibroblasts - فیبروبلاستهای لثهoral squamous cell carcinoma - کارسینوم سلول سنگفرشی دهانیfocal adhesion kinase - کیناز چسبندگی کانونی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Benzimidazole anthelmintics flubendazole and mebendazole are microtubule-targeting drugs that showed considerable anti-cancer activity in different preclinical models. In this study, the effects of flubendazole and mebendazole on proliferation, migration and cadherin switching were studied in a panel of oral cell lines in vitro. Both compounds reduced the viability of the PE/CA-PJ15 and H376 oral squamous carcinoma cells and of the premalignant oral keratinocytes DOK with the IC50 values in the range of 0.19-0.26â¯Î¼M. Normal oral keratinocytes and normal gingival fibroblasts were less sensitive to the treatment. Flubendazole and mebendazole also reduced the migration of the PE/CA-PJ15â¯cell in concentrations that had no anti-migratory effects on the normal gingival fibroblasts. Levels of the focal adhesion kinase FAK, Rho-A and Rac1 GTPases and the Rho guanine nucleotide exchange factor GEF-H1 were decreased in both PE/CA-PJ15â¯cells and gingival fibroblasts following treatment. Both drugs also interfered with cadherin switching in the model of TGF-β-induced epithelial to mesenchymal transition (EMT) in the DOK cell line. Levels of N-cadherin were reduced in the TGF-β induced cells co-treated with flubendazol and mebendazole in very low concentration (50â¯nM). These results suggest direct effects of both benzimidazoles on selected processes of EMT in oral cell lines such as cadherin switching as well as cellular migration.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chemico-Biological Interactions - Volume 293, 25 September 2018, Pages 124-132
Journal: Chemico-Biological Interactions - Volume 293, 25 September 2018, Pages 124-132
نویسندگان
Vera Kralova, Veronika HanuÅ¡ová, KateÅina Caltová, Petr Å paÄek, Martina Hochmalová, Lenka Skálová, Emil Rudolf,